Osteogenic progenitors in bone marrow aspirates from smokers and nonsmokers.
Osteogenic progenitors in bone marrow aspirates from smokers and nonsmokers.
复制标题
骨髓中的成骨祖细胞来自吸烟者和非吸烟者。
DOI：
10.1097/01.blo.0000183738.43245.c2
复制
发表时间：
2006
影响因子：
4.2
通讯作者：
Muschler,GeorgeF
中科院分区：
文献类型：
--
作者：
Bidula,Jennifer;Boehm,Cynthia;Powell,Kimerly;Barsoum,Wael;Nakamoto,Chizu;Mascha,Ed;Muschler,GeorgeF
骨髓抽吸物含有结缔组织祖细胞，其可以增殖以形成表达结缔组织表型的细胞集落。成骨结缔组织祖细胞的数量可以通过计数表达碱性磷酸酶（成骨细胞分化的早期标志物）的集落来估计。由于烟草使用与骨质减少和骨折不愈合有关，我们测试了以下假设：当前或以前的烟草使用是骨髓细胞结构或骨髓源性细胞中成骨结缔组织祖细胞患病率的独立决定因素。从62名患者的髂前嵴获得骨髓穿刺液，这些患者被分为从不吸烟、过去吸烟或目前吸烟者。测定每次抽吸的有核细胞数。将细胞在成骨培养基中培养6天。通过计数集落形成单位确定成骨结缔组织祖细胞的患病率。使用定量图像分析评估每个集落的面积。骨髓细胞密度随年龄增长而降低。我们没有观察到吸烟状态和骨髓细胞，集落患病率，或集落面积之间的关系。这表明，烟草的使用是不相关的骨髓中的成骨结缔组织祖细胞的患病率的变化，或其内在的生物学能力进行早期成骨细胞differentiation.Level的证据：诊断研究，I级（测试先前开发的诊断标准连续患者普遍适用的参考“金”标准）。有关证据等级的完整描述，请参见作者指南。
Bone marrow aspirates contain connective tissue progenitor cells which can proliferate to form cell colonies that express connective tissue phenotypes. The number of osteogenic connective tissue progenitor cells can be estimated by counting colonies that express alkaline phosphatase, an early marker of osteoblastic differentiation. Because tobacco use is associated with decreased bone mass and fracture nonunion, we tested the hypothesis that current or previous tobacco use is an independent determinant of marrow cellularity or prevalence of osteogenic connective tissue progenitor cells among marrow-derived cells. Marrow aspirates were obtained from the anterior iliac crest of 62 patients who were grouped as never smoked, past smokers, or current smokers. The number of nucleated cells per aspirate was determined. Cells were cultured for 6 days in osteogenic media. The prevalence of osteogenic connective tissue progenitor cells was determined by counting colony forming units. The area of each colony was assessed using quantitative image analysis. Cellularity of bone marrow was found to decrease with age. We observed no relationship between smoking status and marrow cellularity, colony prevalence, or colony area. This suggests that tobacco use is not associated with a change in prevalence of osteogenic connective tissue progenitor cells in bone marrow, or their intrinsic biologic capacity to undergo early osteoblastic differentiation.Level of Evidence: Diagnostic Study, Level I (testing of previously developed diagnostic criteria on consecutive patients-with universally applied reference “gold” standard). See the Guidelines for Authors for a complete description of levels of evidence.