Soil core incubation system for the field measurement of denitrification using acetylene-inhibition
Soil core incubation system for the field measurement of denitrification using acetylene-inhibition
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DOI：
10.1016/0038-0717(87)90059-9
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发表时间：
1987
影响因子：
9.7
通讯作者：
J. Rydén;J. Skinner;D. J. Nixon
中科院分区：
文献类型：
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作者：
J. Rydén;J. Skinner;D. J. Nixon
对两种测定反硝化速率的系统进行了比较，这两种系统都基于乙炔抑制技术。一个系统涉及用C2H2在土壤表面放置一个围栏(500×150 mm)来处理土壤，然后测量一氧化二氮(N2O)排放。另一项是在田间将土壤岩心与5%(v/v)的C2H2一起放入改良的水果保鲜罐中培养。对于排水良好的土壤，在广泛的反硝化速率范围内(0.005-1.27kgN ha−1day−1)，两种测量系统之间的一致性很接近。在排水不良、孔隙率低的土壤上获得的结果表明，带土芯的培养系统克服了在这类土壤上应用围隔技术所带来的问题。在适当的时间(通常是24小时)后加入氯仿可以终止孵化系统中的反硝化作用。然后，瓶子空气中的N2O浓度在14天内基本保持不变。这在允许在远离分析设施的地点使用该系统方面提供了第二个优势。
Two systems for determining rates of denitrification, both based on the acetylene-inhibition technique, have been compared. One system involvedin situtreatment of soil with C2H2using an enclosure (500 × 150mm) placed over the soil surface followed by measurement of nitrous oxide (N2O) emission. The other involved incubation, in the field, of soil cores with 5% (v/v) C2H2in modified fruit preserving jars. Agreement between the two systems of measurement was close for well-drained soils over a wide range of rates of denitrification (0.005–1.27 kg N ha−1day−1). Results obtained with poorly-drained soils having low air-filled porosities indicated that the incubation system with soil cores overcame the problems associated with applying the enclosure technique to soils of this type. Denitrification in the incubation system could be terminated by the addition of chloroform after an appropriate period (usually 24 h). The N2O concentration in the air space of the jars then remained essentially constant for 14 days. This provided a second advantage in allowing the system to be used at sites remote from analytical facilities.