Characterization of synthetic-lethal mutants reveals a role for the Saccharomyces cerevisiae guanine-nucleotide exchange factor Cdc24p in vacuole function and Na+ tolerance.
Characterization of synthetic-lethal mutants reveals a role for the Saccharomyces cerevisiae guanine-nucleotide exchange factor Cdc24p in vacuole function and Na+ tolerance.
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合成致死突变体的表征揭示了酿酒酵母鸟嘌呤核苷酸交换因子 Cdc24p 在液泡功能和钠耐受性中的作用。
DOI：
10.1093/genetics/147.1.43
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发表时间：
1997
期刊：
影响因子：
3.3
通讯作者：
Johnson,DI
中科院分区：
文献类型：
--
作者：
White,WH;Johnson,DI
Cdc24p is the guanine-nucleotide exchange factor for the Cdc42p GTPase, which controls cell polarity inSaccharomyces cerevisiae.To identify new genes that may affect cell polarity, we characterized six UV-inducedcsl(CDC24synthetic-lethal) mutants that exhibited synthetic-lethality withcdc24-4tsat 23°. Five mutants were not complemented by plasmid-borneCDC42,RSRI,BUD5,BEM1,BEM2,BEM3orCLA4genes, which are known to play a role in cell polarity. Thecsl3mutant displayed phenotypes similar to those observed with calcium-sensitive, Pet−vmamutants defective in vacuole function.CSL5was allelic toVMA5, the vacuolar H+-ATPase subunit C, and one third ofcs15 cdc24-4tscells were elongated or had misshapen buds. Acdc24-4tsΔvma5::LEU2double mutant did not exhibit synthetic lethality, suggesting that thecsl5/vma5 cdc24-4tssynthetic-lethality was not simply due to altered vacuole function. Thecdc24-4tsmutant, like Δvma5::LEU2andcs13mutants, was sensitive to high levels of Ca2+as well as Na+in the growth media, which did not appear to be a result of a fragile cell wall because the phenotypes were not remedied by 1msorbitol. Our results indicated that Cdc24p was required in one V-ATPase mutant and another mutant affecting vacuole morphology, and also implicated Cdc24p in Na+tolerance.
