The spontaneous activation of a potassium channel during the preparation of resealed human erythrocyte ghosts.
The spontaneous activation of a potassium channel during the preparation of resealed human erythrocyte ghosts.
复制标题
在重新密封的人红细胞血影的制备过程中钾通道的自发激活。
DOI：
10.1016/0005-2736(84)90280-3
复制
发表时间：
1984
期刊：
影响因子：
--
通讯作者：
P. Wood
中科院分区：
文献类型：
--
作者：
P. Wood
发现在耗尽细胞内源螯合剂和代谢物的条件下制备的重新密封的红细胞血影可选择性地渗透钾。钾的净流出受到低浓度外部钾的刺激，并且可以被寡霉素抑制。当在螯合剂或镁存在的情况下通过溶血形成重新密封的鬼影时，不会表现出这种效果。自发激活的途径实际上是钙激活的钾通道，由 Gardos 于 1958 年首次发现。在完整的细胞中，钙泵和内源性螯合剂的联合作用将钙浓度维持在激活阈值以下。目前的观察表明，该通道是由源自细胞本身或培养基中不可避免的钙背景的微量钙自发激活的。耗尽内源性螯合剂的幽灵中的通道表现出对钙的高亲和力。通道激活发生在溶血过程中，并在随后的洗涤过程中持续存在。
Resealed erythrocyte ghosts prepared under conditions which deplete the cell of its endogenous chelators and metabolites are found to be selectively permeable to potassium. The net efflux of potassium is stimulated by low concentrations of external potassium and can be inhibited by oligomycin. The effect is not expressed when resealed ghosts are formed by hemolysis in the presence of chelators or magnesium. The spontaneously activated pathway is actually the calcium-activated potassium channel, first discovered by Gardos in 1958. In the intact cell, the combined actions of the calcium pump and endogenous chelators maintain the calcium concentration below the threshold for activation. Current observations indicate that the channel is spontaneously activated by traces of calcium originating from the cell itself or from the unavoidable background of calcium found in the media. The channel in ghosts depleted of endogenous chelators exhibits its high affinity for calcium. Channel activation occurs during hemolysis and persist throughout subsequent washings.