Low-temperature tolerance and transcriptome analyses during seed germination of Anabasis aphylla
Low-temperature tolerance and transcriptome analyses during seed germination of Anabasis aphylla
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无叶假木贼种子萌发过程的低温耐受性及转录组分析
DOI：
10.1080/17429145.2019.1616840
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发表时间：
2019-01-01
影响因子：
3.2
通讯作者：
Wang, Mei
中科院分区：
文献类型：
--
作者：
Peng, Mengwen;Chang, Yaling;Wang, Mei
本研究旨在利用转录组测序鉴定相关基因，探讨沙漠植物 Anabasis aphylla 在秋季不同种子萌发阶段的低温耐受性差异。不同发芽阶段种子的成活率均随温度降低而降低，各阶段的耐性由大到小依次为：吸胀（I）接近种皮破裂（II）>种皮去除（III）>伸长（IV）。超氧化物歧化酶、过氧化物酶、过氧化氢酶活性和丙二醛含量在第二阶段后呈下降趋势。对种子进行转录组测序，包括成熟和干燥的优势种子 (W1) 以及不同发芽阶段的种子 [吸胀 (W2)、种皮去除 (W3) 和伸长 (W4)]。种子萌发过程中一些基因被下调，主要包括细胞色素P450、油质蛋白、乙烯反应转录因子和低温诱导蛋白。这项研究表明，某些基因的下调可能会导致低温耐受性降低。
This study aimed to explore differences in the low-temperature tolerance of a desert plant, Anabasis aphylla, at different seed germination stages during the fall using transcriptome sequencing to identify related genes. The survival rate of the seeds decreased with lower temperatures at different germination stages, and the tolerance of the stages in decreasing order was as follows: imbibition (I) approximate to testa rupture (II) > testa removal (III) > elongation (IV). Superoxide dismutase, peroxidase, and catalase activities and the malondialdehyde content showed a downward trend after stage II. Transcriptome sequencing of seeds, including ripe and dry dominant seeds (W1) and seeds during different germination stages [imbibition (W2), testa removal (W3), and elongation (W4)] was performed. Some genes were downregulated during seed germination, which mainly included cytochrome P450, oleosin, ethylene-responsive transcription factor, and low temperature-induced protein. This study suggests that downregulated of some genes may result in a decrease in low-temperature tolerance.