EXPRESSION OF ALPHA(2)-MACROGLOBULIN RECEPTOR/LOW DENSITY LIPOPROTEIN RECEPTOR-RELATED PROTEIN AND SCAVENGER RECEPTOR IN HUMAN ATHEROSCLEROTIC LESIONS
EXPRESSION OF ALPHA(2)-MACROGLOBULIN RECEPTOR/LOW DENSITY LIPOPROTEIN RECEPTOR-RELATED PROTEIN AND SCAVENGER RECEPTOR IN HUMAN ATHEROSCLEROTIC LESIONS
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DOI：
10.1172/jci117195
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发表时间：
1994-05-01
影响因子：
15.9
通讯作者：
YLAHERTTUALA, S
中科院分区：
文献类型：
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作者：
LUOMA, J;HILTUNEN, T;YLAHERTTUALA, S
巨噬细胞和平滑肌细胞来源的泡沫细胞是人类动脉粥样硬化病变的典型组成部分。我们用原位杂交和免疫细胞化学方法研究了α 2巨球蛋白受体/低密度脂蛋白受体相关蛋白（α 2 MR/LRP）、清道夫受体和低密度脂蛋白受体在动脉粥样硬化病变中的表达。在人乳腺癌早期和晚期病变的SMC和巨噬细胞中发现了大量的α（2）MR/LRP mRNA和蛋白的表达。正常乳腺癌SMC中也有α 2 MR/LRP表达。损伤巨噬细胞表达清道夫受体mRNA和蛋白，而损伤SMC无表达。LDL受体在病变区不表达，但在靠近外膜边缘的中膜SMC的一些血管中表达。结果表明：（a）α（2）MR/LRP是迄今为止在体内病变SMC中表达的唯一脂蛋白受体;（B）清道夫受体仅在病变巨噬细胞中表达;（c）这两种受体可能在人类动脉粥样硬化病变的发展中起重要作用。
Macrophage- and smooth muscle cell (SMC)-derived foam cells are typical constituents of human atherosclerotic lesions. At least three receptor systems have been characterized that could be involved in the development of foam cells: alpha(2)-macroglobulin receptor/LDL receptor-related protein (alpha(2)MR/LRP), scavenger receptor, and LDL receptor.We studied the expression of these receptors in human atherosclerotic lesions with in situ hybridization and immunocytochemistry. An abundant expression of alpha(2)MR/LRP mRNA and protein was found in SMC and macrophages in both early and advanced lesions in human aortas. alpha(2)MR/LRP was also present in SMC in normal aortas. Scavenger receptor mRNA and protein were expressed in lesion macrophages but no expression was found in lesion SMC. LDL receptor was absent from the lesion area but was expressed in some aortas in medial SMC located near the adventitial border. The results demonstrate that (a) alpha(2)MR/LRP is, so far, the only lipoprotein receptor expressed in lesion SMC in vivo; (b) scavenger receptors are expressed only in lesion macrophages; and (c) both receptors may play important roles in the development of human atherosclerotic lesions.