Activation of the reaper gene during ectopic cell killing in Drosophila
Activation of the reaper gene during ectopic cell killing in Drosophila
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DOI：
10.1006/dbio.1996.0296
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发表时间：
1996-11-25
影响因子：
2.7
通讯作者：
Abrams, JM
中科院分区：
文献类型：
--
作者：
Nordstrom, W;Chen, P;Abrams, JM
reaper（rpr）基因的产物是果蝇中程序性细胞死亡所必需的。我们检测了电离辐射或异常发育引起的异位细胞死亡过程中rpr的表达。在这两种情况下，观察到显着诱导rpr表达。rpr上游的基因组片段赋予lacZ报告基因转基因这种调控行为。在模型细胞培养系统中，REAPER的条件性表达足以诱导大量凋亡，这可以通过抗凋亡蛋白p35来预防。总体而言，这些结果表明，不同的信号会聚在，或上游，rpr相关的转录调控元件，可以发挥作用，启动一个共同的凋亡途径，涉及ICE样蛋白酶活性。(C)出版社：Academic Press，Inc.
The product of the reaper (rpr) gene is required for programmed cell death in Drosophila. We examined rpr expression during ectopic cell deaths caused by ionizing radiation or aberrant development. In both instances, dramatic induction of rpr expression was observed. A genomic fragment upstream of rpr confers this regulatory behavior upon a lacZ reporter transgene. In a model cell culture system, conditional expression of REAPER is sufficient to induce massive apoptosis that can be prevented by the anti-apoptotic protein p35. Overall, these results suggest that diverse signals converge at, or upstream of, rpr-associated transcriptional regulatory elements that can function to initiate a common apoptotic pathway involving ICE-like protease activity. (C) 1996 Academic Press, Inc.