Consumption of galacto-oligosaccharides increases iron absorption from a micronutrient powder containing ferrous fumarate and sodium iron EDTA: a stable-isotope study in Kenyan infants
Consumption of galacto-oligosaccharides increases iron absorption from a micronutrient powder containing ferrous fumarate and sodium iron EDTA: a stable-isotope study in Kenyan infants
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DOI：
10.3945/ajcn.116.145060
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发表时间：
2017-10-01
影响因子：
7.1
通讯作者：
Zimmermann, Michael B.
中科院分区：
文献类型：
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作者：
Paganini, Daniela;Uyoga, Mary A.;Zimmermann, Michael B.
背景资料：益生元的摄入是否会增加婴儿的铁吸收尚不清楚。目的：我们确定益生元的摄入是否会影响含有富马酸亚铁和EDTA铁钠混合物的微量营养素粉（MNP）中的铁吸收（FeFum+NaFeEDTA）在肯尼亚婴儿中的应用。设计：婴儿（n = 50;年龄6-14月龄）食用了用含有FeFum+NaFeEDTA和7.5 g低聚半乳糖（GOSs）的MNP强化的玉米粥铁组（n = 28）每天给予相同的MNP（铁+GOS组，n = 22）或不加GOS的MNP（铁组，n = 28），共3 wk。然后，连续2天，我们给所有婴儿喂食同位素标记的玉米粥和含有5 mg Fe的MNP测试餐（57）FeFum+Na-58 FeEDTA或硫酸亚铁（（FeSO 4）-Fe-54）。铁吸收被测量为稳定同位素的红细胞掺入。在基线和第3周评估铁标记物、粪便pH值和细菌群。组内和组间的比较与使用mixed-effects models.Results：有一个显着的组由化合物的相互作用对铁的吸收（P = 0.011）。Fe组从FeFum+NaFeEDTA和从FeSO 4中吸收的分数铁的中位百分比为11.6%（IQR：6.9-19.9%）和20.3% Fe+GOS组分别为18.8%（IQR：8.3-37.5%）和25.5%（IQR：15.1-37.8%）（P = 0.124）。两组间，Fe+GOS组中FeFum+NaFeEDTA的铁吸收更大（P = 0.047），但FeSO 4的铁吸收不大（P = 0.653）。与FeSO 4相比，FeFum+NaFeEDTA的相对铁生物利用度在Fe+GOS组中高于Fe组（88%与63%; P = 0.006）。对双歧杆菌属存在显著的时间-组相互作用。（P = 0.008）和乳杆菌/片球菌/明串珠菌属（Leuconostoc spp.）（P = 0.018）;乳杆菌/片球菌/明串珠菌属;铁组中双歧杆菌减少（P = 0.013），双歧杆菌属的数量增加的趋势不显著。Fe+GOS组（P = 0.099）。3周时，铁吸收与粪便pH值呈负相关（P < 0.001），与乳酸杆菌/片球菌/明串珠菌呈正相关（P < 0.001）。结论：婴儿摄入GOS使含FeFum+NaFeEDTA的MNP的铁吸收增加了62%，从而可能反映了更大的结肠铁吸收。本试验在clinicaltrials.gov上注册为NCT 02666417。
Background: Whether consumption of prebiotics increases iron absorption in infants is unclear.Objective: We set out to determine whether prebiotic consumption affects iron absorption from a micronutrient powder (MNP) containing a mixture of ferrous fumarate and sodium iron EDTA (FeFum+NaFeEDTA) in Kenyan infants.Design: Infants (n = 50; aged 6-14 mo) consumed maize porridge that was fortified with an MNP containing FeFum+NaFeEDTA and 7.5 g galacto-oligosaccharides (GOSs) (Fe+GOS group, n = 22) or the same MNP without GOSs (Fe group, n = 28) each day for 3 wk. Then, on 2 consecutive days, we fed all infants isotopically labeled maize porridge and MNP test meals containing 5 mg Fe as (57)FeFum+Na-58 FeEDTA or ferrous sulfate ((FeSO4)-Fe-54). Iron absorption was measured as the erythrocyte incorporation of stable isotopes. Iron markers, fecal pH, and bacterial groups were assessed at baseline and 3 wk. Comparisons within and between groups were done with the use of mixed-effects models.Results: There was a significant group-by-compound interaction on iron absorption (P = 0.011). The median percentages of fractional iron absorption from FeFum+NaFeEDTA and from FeSO4 in the Fe group were 11.6% (IQR: 6.9-19.9%) and 20.3% (IQR: 14.2-25.7%), respectively, (P < 0.001) and, in the Fe+GOS group, were 18.8% (IQR: 8.3-37.5%) and 25.5% (IQR: 15.1-37.8%), respectively (P = 0.124). Between groups, iron absorption was greater from the FeFum+NaFeEDTA (P = 0.047) in the Fe+GOS group but not from the FeSO4 (P = 0.653). The relative iron bioavailability from FeFum+NaFeEDTA compared with FeSO4 was higher in the Fe+GOS group than in the Fe group (88% compared with 63%; P = 0.006). There was a significant time-by-group interaction on Bifidobacterium spp. (P = 0.008) and Lactobacillus/Pediococcus/Leuconostoc spp. (P = 0.018); Lactobacillus/Pediococcus/Leuconostoc spp. decreased in the Fe group (P = 0.013), and there was a nonsignificant trend toward higher Bifidobacterium spp. in the Fe+GOS group (P = 0.099). At 3 wk, iron absorption was negatively correlated with fecal pH (P < 0.001) and positively correlated with Lactobacillus/Pediococcus/Leuconostoc spp. (P = 0.001).Conclusion: GOS consumption by infants increased iron absorption by 62% from an MNP containing FeFum+NaFeEDTA, thereby possibly reflecting greater colonic iron absorption. This trial was registered at clinicaltrials.gov as NCT02666417.