An iron-binding exochelin prevents restenosis due to coronary artery balloon injury in a porcine model.
An iron-binding exochelin prevents restenosis due to coronary artery balloon injury in a porcine model.
复制标题
铁结合外螯素可预防猪模型中冠状动脉球囊损伤引起的再狭窄。
DOI：
10.1161/hc4301.097194
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发表时间：
2001
期刊：
影响因子：
37.8
通讯作者：
Horwitz,LD
中科院分区：
文献类型：
--
作者：
Rosenthal,EA;Bohlmeyer,TJ;Monnet,E;MacPhail,C;Robertson,AD;Horwitz,MA;Burchenal,JE;Horwitz,LD
背景血管平滑肌细胞（VSMC）增殖是冠状动脉成形术（PTCA）后新内膜形成导致再狭窄的关键因素。Desferri-exochelin 772SM （D-EXO）是一种由结核分枝杆菌分泌的高度扩散的亲脂性铁螯合剂，在培养中抑制VSMCs的增殖。我们假设用D-EXO治疗可以在体内抑制球囊损伤血管的新内膜形成。方法和结果我们用标准PTCA球囊对24头猪进行冠状动脉过伸损伤，然后通过浸润导管给药D-EXO （n=14）或载药（n=10）。治疗是随机的，研究者对治疗组采取盲法，直到数据分析完成。1个月后，我们对猪实施安乐死，切除损伤的冠状动脉节段，对每节段进行多次切片，并确定最大新生内膜形成的部位。根据内部或外部弹性板或介质的破坏程度进行损伤评分。D-EXO将损伤评分调整后的狭窄指数（新生内膜面积除以内弹性板面积）降低了47%。新生内膜厚度也有所减少。结论sd - exo可明显抑制猪血管损伤模型中新生内膜的形成。
BackgroundVascular smooth muscle cell (VSMC) proliferation is a critical factor in the neointima formation that causes restenosis after coronary angioplasty (PTCA). Desferri-exochelin 772SM (D-EXO), a highly diffusible, lipophilic iron chelator secreted byMycobacterium tuberculosis, inhibits proliferation of VSMCs in culture. We hypothesized that treatment with D-EXO would inhibit neointima formation in balloon-injured vessels in vivo.Methods and ResultsWe subjected 24 pigs to overstretch coronary artery injury with standard PTCA balloons and then administered intramural injections of either D-EXO (n=14) or vehicle (n=10) through an Infiltrator catheter. Treatments were randomized, and the investigators were blinded with regard to treatment group until data analysis was completed. One month later, we euthanized the pigs, excised the injured coronary segments, made multiple sections of each segment, and identified the site of maximal neointima formation. An injury score based on the degree of disruption of the internal or external elastic lamina or media was assigned. D-EXO reduced stenosis index (neointima area divided by the area within the internal elastic lamina), adjusted for injury score, by 47%. Neointima thickness was also reduced.ConclusionsD-EXO, injected intramurally, substantially inhibited formation of neointima in a porcine vascular injury model.