Transcriptional responses to loss of RNase H2 in Saccharomyces cerevisiae.
Transcriptional responses to loss of RNase H2 in Saccharomyces cerevisiae.
复制标题
酿酒酵母中RNase H2损失的转录反应。
DOI：
10.1016/j.dnarep.2012.09.006
复制
发表时间：
2012-12-01
期刊：
影响因子：
3.8
通讯作者：
Kunkel, Thomas A.
中科院分区：
文献类型：
--
作者：
Arana, Mercedes E.;Kerns, Robnet T.;Wharey, Laura;Gerrish, Kevin E.;Bushel, Pierre R.;Kunkel, Thomas A.
我们在这里报告的转录反应，在酿酒酵母RNH 201基因编码的催化亚基的RNase H2的删除。RNH 201的缺失改变了349个基因的RNA表达≥1.5倍（q值<0.01），其中123个上调，226个下调。差异表达基因（DEG）包括参与应激反应和基因组维持的基因，这与RNA酶H2在复制过程中去除掺入DNA中的核糖核苷酸的作用一致。上调的基因包括编码RNA聚合酶I和III的亚基的几个基因，以及参与核糖体RNA加工、核糖体生物发生和tRNA修饰和加工的基因，支持RNA酶H2在解析rRNA和tRNA基因转录期间形成的R环中的作用。与上调基因相比，下调基因的转录起始位点附近的平均GC含量更高，进一步表明了在R环解析中的作用。几个DEG参与端粒维持，支持RNA酶H2在解决端粒处形成的RNA-DNA杂交中的作用。大量DEG编码参与应答dsRNA病毒的核酸酶、解旋酶和基因，观察结果可能与在RNA酶H2缺陷型人类中引发先天免疫应答的核酸种类相关。
We report here the transcriptional responses in Saccharomyces cerevisiae to deletion of the RNH201 gene encoding the catalytic subunit of RNase H2. Deleting RNH201 alters RNA expression of 349 genes by ≥1.5-fold (q-value <0.01), of which 123 are upregulated and 226 are downregulated. Differentially expressed genes (DEGs) include those involved in stress responses and genome maintenance, consistent with a role for RNase H2 in removing ribonucleotides incorporated into DNA during replication. Upregulated genes include several that encode subunits of RNA polymerases I and III, and genes involved in ribosomal RNA processing, ribosomal biogenesis and tRNA modification and processing, supporting a role for RNase H2 in resolving R-loops formed during transcription of rRNA and tRNA genes. A role in R-loop resolution is further suggested by a higher average GC-content proximal to the transcription start site of downregulated as compared to upregulated genes. Several DEGs are involved in telomere maintenance, supporting a role for RNase H2 in resolving RNA-DNA hybrids formed at telomeres. A large number of DEGs encode nucleases, helicases and genes involved in response to dsRNA viruses, observations that could be relevant to the nucleic acid species that elicit an innate immune response in RNase H2-defective humans.
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影响因子：
56.9
作者：
Azzalin, Claus M.;Reichenbach, Patrick;Lingner, Joachim
通讯作者：
Lingner, Joachim
影响因子：
30.8
作者：
Crow, Yanick J.;Leitch, Andrea;Jackson, Andrew P.
通讯作者：
Jackson, Andrew P.
影响因子：
14.8
作者：
Huang, Da Wei;Sherman, Brad T.;Lempicki, Richard A.
通讯作者：
Lempicki, Richard A.
影响因子：
3.8
作者：
Clark AB;Lujan SA;Kissling GE;Kunkel TA
通讯作者：
Kunkel TA
DOI：
10.1073/pnas.92.8.3526
发表时间：
1995-04-11
影响因子：
11.1
作者：
DROLET, M;PHOENIX, P;CROUCH, RJ
通讯作者：
CROUCH, RJ