LIPID-COMPOSITION AND ACID HYDROLASE CONTENT OF LAMELLAR GRANULES OF FETAL-RAT EPIDERMIS
LIPID-COMPOSITION AND ACID HYDROLASE CONTENT OF LAMELLAR GRANULES OF FETAL-RAT EPIDERMIS
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DOI：
10.1111/1523-1747.ep12276831
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发表时间：
1985-01-01
影响因子：
6.5
通讯作者：
TRACZYK, TN
中科院分区：
文献类型：
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作者：
FREINKEL, RK;TRACZYK, TN
脂质和酸性水解酶已被鉴定为富含板层颗粒（LG）的亚细胞组分，来自胎鼠表皮。该馏分含有23%的葡萄糖基神经酰胺和神经酰胺，15%的游离甾醇和34%的磷脂。液体/蛋白质比例为2.0。甾醇和鞘脂的存在比例与先前报告的角质层中的比例相似。这些发现提供了直接的生化证据，广泛接受的假设，角质层脂质来自胞吐作用的层状颗粒进入细胞间隙。LG级分富含某些酸性水解酶，包括葡糖苷酶、酸性磷酸酶、磷脂酶A和鞘磷脂酶;其他酸性水解酶，即，氨基糖苷酶，半乳糖苷酶和芳基硫酸酯酶（pH 5.5），和类固醇硫酸酯酶没有优先定位在这一部分。通过调节角质层膜区域的磷脂、糖脂和蛋白质，LG的酸性水解酶可能在角质层的功能和脱屑中发挥相关作用。
Lipids and acid hydrolases have been characterizied in a subcelluar fraction, enriched with lamellar granules (LG), derived from fetal rat epidermis. This fraction contains 23% glucosyl ceramides and ceramides, 15% free sterols, and 34% phospholipids. The liquid/protein ratio is 2.0. The sterols and sphingolipids were present in proportions similar to those previously reported in stratum corneum. These findings provide direct biochemical evidence for the widely accepted hypothesis that stratum corneum lipids are derived from exocytosis of lamellar granules into the intercellular space. The LG fraction was enriched in certain acid hydrolases including glucosidase, acid phosphatase, phospholipases A, and sphingomyelinase; other acid hydrolases, i.e., aminoglycosidases, glactosidase and aryl sulfatase (pH 5.5), and steroid sulfatase were not preferentially localized in this fraction. By modulation of phospholipids, glycolipids, and proteins in the membrane regions of stratum corneum, the acid hydrolases of LG may play a role relevant to the function and desquamation of stratum corneum.