Involvement of the Nuclear Progestin Receptor in LH-induced Expression of Membrane Type 2 Matrix Metalloproteinase Required for Follicle Rupture during Ovulation in the Medaka, Oryzias latipes.
Involvement of the Nuclear Progestin Receptor in LH-induced Expression of Membrane Type 2 Matrix Metalloproteinase Required for Follicle Rupture during Ovulation in the Medaka, Oryzias latipes.
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核孕激素受体参与 LH 诱导的青鳉、Oryzias latipes 排卵期间卵泡破裂所需的膜 2 型基质金属蛋白酶的表达。
DOI：
10.1016/j.mce.2017.04.016
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
T.
中科院分区：
文献类型：
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作者：
Ogiwara;K.;Takahashi;T.
研究了激素对硬骨鱼排卵不可或缺的蛋白水解酶Mmp15表达的调节。在利用排卵前卵泡的体外培养系统中，重组黄体生成素（rLh）的存在诱导了Mmp15的表达和排卵。rlh诱导的Mmp15表达和排卵均依赖于17α， 20β-二羟基-4-孕激素-3- 1，提示核黄体酮受体（Pgr）的参与。Pgr拮抗剂RU-486可降低体外卵泡排卵和Mmp15的表达。与Pgr一样，转录因子CCAAT/增强子结合蛋白β (Cebpb)也被rLh诱导。ChIP分析表明，Pgr和Cebpb结合到mmp15启动子区域。这些结果表明rlh诱导的Mmp15的表达是由Pgr和Cebpb介导的。促排卵前卵泡中Pgr和Cebpb的不同表达时间似乎解释了Pgr基因激活到mp15基因表达之间相当长的时滞。
Hormonal regulation of the expression of Mmp15, a proteolytic enzyme indispensable for ovulation in the teleost medaka, was investigated. In an in vitro culture system using preovulatory follicles, Mmp15 expression and ovulation were induced in the presence of recombinant luteinizing hormone (rLh). Both rLh-induced Mmp15 expression and ovulation were 17α, 20β-dihydroxy-4-pregnen-3-one-dependent, suggesting the involvement of a nuclear progestin receptor (Pgr). In vitro follicle ovulation and Mmp15 expression were reduced by treatment with the Pgr antagonist RU-486. Like Pgr, the transcription factor CCAAT/enhancer-binding protein β (Cebpb) was induced by rLh. ChIP analyses indicated that Pgr and Cebpb bound to themmp15promoter region. These results indicate that the rLh-induced expression of Mmp15 is mediated by Pgr and Cebpb. A differential timing of expression of Pgr and Cebpb in the preovulatory follicles appears to explain the considerably long time-lag from thepgrgene activation tommp15gene expression.