Synthesis of Triazole-Linked Oligonucleotides with High Affinity to DNA Complements and an Analysis of Their Compatibility with Biosystems
Synthesis of Triazole-Linked Oligonucleotides with High Affinity to DNA Complements and an Analysis of Their Compatibility with Biosystems
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DOI：
10.1021/jo400651k
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发表时间：
2013-06-21
影响因子：
3.6
通讯作者：
Florentiev, Vladimir L.
中科院分区：
文献类型：
--
作者：
Varizhuk, Anna M.;Kaluzhny, Dmitry N.;Florentiev, Vladimir L.
合成了具有三唑核苷酸间连接的新型寡核苷酸类似物，并研究了它们的杂交性质。这些类似物表现出与未经修饰的寡核苷酸相似的DNA结合亲和力。这种修饰可以保护寡核苷酸不受核酸酶的影响。将修饰后的寡核苷酸作为聚合酶链式反应的引物进行检测。远离3‘末端的修饰是聚合酶所能容忍的。我们的结果表明，这些新的寡核苷酸类似物是迄今为止最有希望的三氮唑DNA模拟物之一。
New oligonucleotide analogues with triazole internucleotide linkages were synthesized, and their hybridization properties were studied. The analogues demonstrated DNA binding affinities similar to those of unmodified oligonucleotides. The modification was shown to protect the oligonucleotides from nuclease hydrolysis. The modified oligonucleotides were tested as PCR primers. Modifications remote from the 3'-terminus were tolerated by polymerases. Our results suggest that these new oligonucleotide analogues are among the most promising triazole DNA mimics characterized to date.