Partnership of Arthrobacter and Pimelobacter in Aerobic Degradation of Sulfadiazine Revealed by Metagenomics Analysis and Isolation
Partnership of Arthrobacter and Pimelobacter in Aerobic Degradation of Sulfadiazine Revealed by Metagenomics Analysis and Isolation
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DOI：
10.1021/acs.est.7b05913
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发表时间：
2018-03-06
影响因子：
11.4
通讯作者：
Zhang, Tong
中科院分区：
文献类型：
--
作者：
Deng, Yu;Wang, Yulin;Zhang, Tong
在这项研究中，宏基因组分析相结合的培养为基础的技术作为一种嵌套的方法，以确定功能显着的细菌富集社区内的磺胺嘧啶生物降解。宏基因组研究表明，我们以前分离的磺胺嘧啶降解菌，节杆菌属D2，和另一种Pimelabacterium细菌伴随发生，作为最丰富的成员在社区的富集文化，进行了超过两年的磺胺嘧啶完全矿化。富集种群对单一碳源交替的响应进一步表明了该Pimelcite成员在磺胺嘧啶最突出的中间代谢产物2-氨基嘧啶上生长的能力。利用这种倾向，额外的培养程序成功分离了Pimelobacter sp. LG 209，其基因组序列与磺胺嘧啶富集培养物中的优势Pimelobacter细菌完全匹配。整合宏基因组调查与分离株的生理特征最终证明，磺胺嘧啶矿化在一个长期运行的富集培养物显着介导的主要磺胺嘧啶降解的专业菌株节杆菌属D2与2-氨基嘧啶降解的合作伙伴菌株Pimelobacter属LG 209。在这里，我们提供了稳定的磺胺嘧啶矿化过程中的微生物相互作用的第一个机制的见解，这将有助于制定适当的磺胺嘧啶污染的热点地区的生物修复策略。
In this study, metagenomic analyses were combined with cultivation-based techniques as a nested approach to identify functionally significant bacteria for sulfadiazine biodegradation within enrichment communities. The metagenomic investigations indicated that our previously isolated sulfadiazine degrader, Arthrobacter sp. D2, and another Pimelobacter bacterium concomitantly occurred as most abundant members in the community of an enrichment culture that performed complete sulfadiazine mineralization for over two years. Responses of the enriched populations to sole carbon source alternation further suggested the ability of this Pimelobacter member to grow on 2-aminopyrimidine, the most prominent intermediate metabolite of sulfadiazine. Taking advantage of this propensity, additional cultivation procedures have enabled the successful isolation of Pimelobacter sp. LG209, whose genomic sequences exactly matched that of the dominant Pimelobacter bacterium in the sulfadiazine enrichment culture. Integration of metagenomic investigations with the physiological characteristics of the isolates conclusively demonstrated that the sulfadiazine mineralization in a long-running enrichment culture was prominently mediated by primary sulfadiazine-degrading specialist strain Arthrobacter sp. D2 in association with the 2-aminopyrimidine-degrading partner strain Pimelobacter sp. LG209. Here, we provided the first mechanistic insight into microbial interactions in steady sulfadiazine mineralization processes, which will help develop appropriate bioremediation strategies for sulfadiazine-contaminated hotspot sites.