Effects of velvet antler polypeptide on sexual behavior and testosterone synthesis in aging male mice.
Effects of velvet antler polypeptide on sexual behavior and testosterone synthesis in aging male mice.
复制标题
鹿茸多肽对老龄雄性小鼠性行为及睾酮合成的影响
DOI：
10.4103/1008-682x.166435
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发表时间：
2016-07
影响因子：
2.9
通讯作者：
Deng CH
中科院分区：
文献类型：
--
作者：
Zang ZJ;Tang HF;Tuo Y;Xing WJ;Ji SY;Gao Y;Deng CH
采用24月龄血清睾酮水平低的雄性C57BL/6小鼠作为迟发性性腺功能减退（LOH）动物模型，研究鹿茸多肽（VAP）对性功能和睾酮合成的影响。这些小鼠通过每天灌胃100、200或300 mg kg - 1体重剂量的VAP连续5周（n = 10只小鼠/剂量）。对照动物（n = 10）给予相同的以重量为基础的载具体积。末次给药后测定各组性行为及血清和睾丸间质组织睾酮水平。此外，为了研究VAP在体内影响性行为和睾酮合成的机制，我们还通过免疫荧光染色和实时荧光定量PCR检测了睾丸间质细胞中类固醇急性调节蛋白（StAR）、细胞色素P450胆固醇侧链切割酶（P450scc）和3β-羟基类固醇脱氢酶（3β-HSD）的表达。结果，VAP显著改善了这些衰老雄性小鼠的性功能。vap治疗组血清睾酮水平和睾丸内睾酮（ITT）浓度均升高。与对照组相比，vap处理组的StAR、P450scc和3β-HSD的表达也有所增强。我们的研究结果表明，VAP可以有效地改善衰老雄性小鼠的性功能。鹿茸对性功能的影响是由于增加了几种睾酮合成限速酶（StAR、P450scc和3β-HSD）的表达，从而促进了体内睾酮的合成。
Twenty-four-month-old male C57BL/6 mice with low serum testosterone levels were used as a late-onset hypogonadism (LOH) animal model for examining the effects of velvet antler polypeptide (VAP) on sexual function and testosterone synthesis. These mice received VAP for 5 consecutive weeks by daily gavage at doses of 100, 200, or 300 mg kg−1 body weight per day (n = 10 mice per dose). Control animals (n = 10) received the same weight-based volume of vehicle. Sexual behavior and testosterone levels in serum and interstitial tissue of testis were measured after the last administration of VAP. Furthermore, to investigate the mechanisms of how VAP affects sexual behavior and testosterone synthesis in vivo, the expression of steroidogenic acute regulatory protein (StAR), cytochrome P450 cholesterol side-chain cleavage enzyme (P450scc), and 3β-hydroxysteroid dehydrogenase (3β-HSD) in Leydig cells was also measured by immunofluorescence staining and quantitative real-time PCR. As a result, VAP produced a significant improvement in the sexual function of these aging male mice. Serum testosterone level and intratesticular testosterone (ITT) concentration also increased in the VAP-treated groups. The expression of StAR, P450scc, and 3β-HSD was also found to be enhanced in the VAP-treated groups compared with the control group. Our results suggested that VAP was effective in improving sexual function in aging male mice. The effect of velvet antler on sexual function was due to the increased expression of several rate-limiting enzymes of testosterone synthesis (StAR, P450scc, and 3β-HSD) and the following promotion of testosterone synthesis in vivo.