ISWI is an ATP-dependent nucleosome remodeling factor
ISWI is an ATP-dependent nucleosome remodeling factor
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DOI：
10.1016/s1097-2765(00)80314-7
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发表时间：
1999-02-01
期刊：
影响因子：
16
通讯作者：
Becker, PB
中科院分区：
文献类型：
--
作者：
Corona, DFV;Längst, G;Becker, PB
ATP酶ISWI是几种不同的核小体重塑复合物的亚基，其增加染色质中DNA的可及性。我们发现，分离的ISWI蛋白本身能够进行核小体重塑，核小体重排和染色质组装反应。核小体对ISWI的ATP酶活性有明显的刺激作用，而游离DNA和游离组蛋白对ISWI的ATP酶活性无明显影响，表明ISWI识别核小体的一种特殊结构特征。因此，核小体重塑不需要ISWI和ISWI复合物的其他亚基之间的功能性相互作用。与ISWI相关的蛋白质的作用可能是调节重塑引擎的活性或定义核小体重塑反应发生的生理背景。
The ATPase ISWI is a subunit of several distinct nucleosome remodeling complexes that increase the accessibility of DNA in chromatin. We found that the isolated ISWI protein itself was able to carry out nucleosome remodeling, nucleosome rearrangement, and chromatin assembly reactions. The ATPase activity of ISWI was stimulated by nucleosomes but not by free DNA or free histones, indicating that ISWI recognizes a specific structural feature of nucleosomes. Nucleosome remodeling, therefore, does not require a functional interaction between ISWI and the other subunits of ISWI complexes. The role of proteins associated with ISWI may be to regulate the activity of the remodeling engine or to define the physiological context within which a nucleosome remodeling reaction occurs.