Colocalization of insulin and glucagon in insulinoma cells and developing pancreatic endocrine cells
Colocalization of insulin and glucagon in insulinoma cells and developing pancreatic endocrine cells
复制标题
胰岛素和胰高血糖素在胰岛素瘤细胞和发育中的胰腺内分泌细胞中的共定位
DOI：
10.1016/j.bbrc.2015.04.072
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发表时间：
2015-06-12
影响因子：
3.1
通讯作者：
Lou, Jinning
中科院分区：
文献类型：
--
作者：
Wang, Zai;You, Jia;Lou, Jinning
人类和大鼠胰岛素瘤的很大一部分共表达多种激素。在一些早期胰腺内分泌细胞中也观察到这种称为多激素的特征，这些细胞共表达胰岛素和胰高血糖素，表明这两种细胞的分化状态不完全。本研究表明，胰岛素瘤细胞INS-1和INS-1衍生的单细胞克隆INS-1-15在部分细胞中共表达胰岛素和胰高血糖素。这两种激素高度集中于细胞内的囊泡中。由于ins -1衍生细胞中同时存在PC1/3和PC2，因此胰高血糖素原可以被加工成胰高血糖素、GLP-1和GLP-2。这些胰高血糖素家族肽和胰岛素同时分泌，与葡萄糖浓度升高相对应。在大鼠胎儿胰腺内分泌细胞中也观察到胰岛素和胰高血糖素的共表达和部分共定位，但共定位率普遍较低且多样性更强，提示在发育中的胰腺内分泌细胞中，胰岛素和胰高血糖素可能储存在不同的分泌囊泡池中，在刺激下可能不一致地分泌。(C) 2015爱思唯尔公司版权所有。
A significant portion of human and rat insulinomas coexpress multiple hormones. This character termed as multihormonality is also observed in some early pancreatic endocrine cells which coexpress insulin and glucagon, suggesting an incomplete differentiation status of both cells. Here we demonstrate that insulinoma cells INS-1 and INS-1-derived single cell clone INS-1-15 coexpressed insulin and glucagon in a portion of cells. These two hormones highly colocalized in the intracellular vesicles within a cell. Due to the existence of both PC1/3 and PC2 in INS-1-derived cells, proglucagon could be processed into glucagon, GLP-1 and GLP-2. These glucagon-family peptides and insulin were secreted simultaneously corresponding to the elevating glucose concentrations. The coexpression and partial colocalization of insulin and glucagon was also observed in rat fetal pancreatic endocrine cells, but the colocalization rate was generally lower and more diverse, suggesting that in the developing pancreatic endocrine cells, insulin and glucagon may be stored in nonidentical pools of secreting vesicles and might be secreted discordantly upon stimulus. (C) 2015 Elsevier Inc. All rights reserved.