Reciprocal effects of glucose on the process of cell death induced by calcium ionophore or H2O2 in rat lymphocytes
Reciprocal effects of glucose on the process of cell death induced by calcium ionophore or H2O2 in rat lymphocytes
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DOI：
10.1016/j.tox.2006.05.004
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发表时间：
2006-08-15
期刊：
影响因子：
4.5
通讯作者：
Oyama, Yasuo
中科院分区：
文献类型：
--
作者：
Horimoto, Kanna;Nishimura, Yumiko;Oyama, Yasuo
我们研究了高浓度葡萄糖对细胞内Ca2+浓度过度增加（[Ca2+](i)）或大鼠淋巴细胞氧化应激诱导的细胞死亡过程的影响。过度增加[Ca2+](i)引起的细胞死亡似乎是由Ca2+，依赖K+通道的激活诱导的，因为Ca2+依赖K+通道的抑制剂减轻了细胞活力的下降。葡萄糖在30-50 mM时通过过量增加[Ca2+]增强了细胞活力的下降(i)。它不是针对葡萄糖的，因为它是针对蔗糖或NaCl的，这表明葡萄糖的不良反应与渗透压升高有关。相反，葡萄糖对H2O2（一种活性氧）诱导的氧化应激细胞有保护作用。果糖和蔗糖也是如此，但NaCl则不然。H2O2诱导细胞死亡的过程开始，不依赖于葡萄糖的存在。葡萄糖延缓H2O2诱导的细胞死亡过程。蔗糖和果糖也能保护细胞免受氧化应激。蔗糖对活性氧的反应活性低于葡萄糖和果糖。反应性的顺序不能解释葡萄糖的保护作用。高浓度的葡萄糖在氧化应激和[Ca2+]过度增加诱导的细胞死亡过程中发挥相互作用。2006爱思唯尔爱尔兰有限公司版权所有。
We have examined the effects of glucose at high concentrations on the process of cell death induced by excessive increase in intracellular Ca2+ concentration ([Ca2+](i)) or oxidative stress in rat lymphocytes. The cell death elicited by the excessive increase in [Ca2+](i) seemed to be induced by an activation of Ca2+,-dependent K+ channels because the inhibitors for Ca2+-dependent K+ channels attenuated the decrease in cell viability. Glucose at 30-50 mM augmented the decrease in cell viability by the excessive increase in [Ca2+](i). It was not specific for glucose because it was the case for sucrose or NaCl, suggesting an involvement of increased osmolarity in adverse action of glucose. On the contrary, glucose protected the cells suffering from oxidative stress induced by H2O2, one of reactive oxygen species. It was also the case for fructose or sucrose, but not for NaCl. The process of cell death induced by H2O2 started, being independent from the presence of glucose. Glucose delayed the process of cell death induced by H2O2. Sucrose and fructose also protected the cells against oxidative stress. The reactivity of sucrose to reactive oxygen species is lower than those of glucose and fructose. The order in the reactivity cannot explain the protective action of glucose. Glucose at high concentrations exerts reciprocal actions on the process of cell death induced by the oxidative stress and excessive increase in [Ca2+],. (c) 2006 Elsevier Ireland Ltd. All rights reserved.