Use of a steroid cyclic disulfide anchor in constructing gold nanoparticle-oligonucleotide conjugates
Use of a steroid cyclic disulfide anchor in constructing gold nanoparticle-oligonucleotide conjugates
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DOI：
10.1021/bc990152n
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发表时间：
2000-03-01
影响因子：
4.7
通讯作者：
Mirkin, CA
中科院分区：
文献类型：
--
作者：
Letsinger, RL;Elghanian, R;Mirkin, CA
描述了一种新的锚定基团，用于将寡核苷酸结合到金表面。基于4，5-二羟基-1，2-二硫烷和表雄酮衍生的缩酮，它易于制备并与寡核苷酸连接。用这种环状二硫化物连接物制备的金纳米颗粒-寡核苷酸结合物是检测特定寡核苷酸序列的有效探针，并且它们对二硫苏糖醇的稳定性比用传统的巯基己基或无环二硫单元制备的相应结合物要大得多。对硫醇失活的高度稳定性可能至少部分原因是通过两个硫原子将每个寡核苷酸锚定在金上。
A new anchoring group is described for binding oligonucleotides to gold surfaces. On the basis of a ketal derived from 4,5-dihydroxy-1,2 dithiane and epiandrosterone, it is easy to prepare and to link to oligonucleotides. Gold nanoparticle-oligonucleotide conjugates made using this cyclic disulfide linker serve as effective probes far detecting specific oligonucleotide sequences, and they exhibit much greater stability toward dithiothreitol than corresponding conjugates prepared with the conventional mercaptohexyl group or an acyclic disulfide unit. The high stability toward thiol deactivation likely results, in part at least, from anchoring each oligonucleotide to gold through two sulfur atoms.