Effect of prolonged melatonin administration on metabolic parameters and organ weights in young male and female Sprague-Dawley rats
Effect of prolonged melatonin administration on metabolic parameters and organ weights in young male and female Sprague-Dawley rats
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DOI：
10.2754/avb200372020163
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发表时间：
2003-06-01
影响因子：
0.6
通讯作者：
Ahlers, I
中科院分区：
文献类型：
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作者：
Marková, M;Adámeková, E;Ahlers, I
引入褪黑激素作为药物的问题仍然悬而未决。特别是长期服用褪黑素需要进行分析和讨论。本研究的目的是分析低剂量褪黑素（4杯/毫升自来水）给药70天，每天15.00至08.00 h（08.00至15.00 h动物饮用自来水）对雄性和雌性Sprague-Dawley大鼠选定代谢参数的影响。除了记录血清和组织中甘油三酯、磷脂、胆固醇、丙二醛、葡萄糖和糖原、血清皮质酮和胰岛素的浓度/含量外，还记录了选定器官的重量、围膜和附睾脂肪以及体重。对照组不给褪黑素。在给药前和给药后9周进行口服葡萄糖耐量试验。将5周龄的雄性和雌性大鼠适应于标准的室内条件和人工光照方案（L:D- 12:12 h），饲喂脂肪含量至少为2.5%的MP饲粮，并随意饮用自来水或褪黑素溶液。每周给大鼠称重两次，并记录食物和水的摄入量。10周后处死，称重各脏器和组织，测定血清、肝脏、心肌和骨髓（股骨）中上述代谢参数。褪黑素可显著降低雄性大鼠血清甘油三酯浓度和肝糖原含量，提高雌性大鼠肝脏磷脂含量。褪黑素没有明显改变其他代谢参数的值。从试验第19天至试验结束，延长褪黑素给药时间显著增加了雌性大鼠心肌和外周血脂肪的重量，显著降低了雄性大鼠肾上腺、肝脏、心肌和附睾脂肪的重量及体重。饮酒女性的体重与对照组相似。饮用mel的男性的水和食物摄入量与对照组没有差异，女性在第6周暂时增加。长期服用褪黑素对血糖水平没有显著影响。给药前口服糖耐量试验曲线正常；给药9周后，女性组（9例）出现1条异常曲线，男性组（9例）出现2条异常曲线。长期褪黑素治疗导致体重、附睾和外周脂肪以及心肌重量的性别差异。
The question of the introduction of melatonin as a drug remains still open. Especially the long term melatonin administration have to be analysed and discussed. The aim of present study was to analyze the effect of low doses of melatonin (4 mug/ml of tap water) administered for 70 days, daily from 15.00 to 08.00 h (from 08.00 to 15.00 h animals were drinking tap water) on selected metabolic parameters in male and female Sprague-Dawley rats. In addition to concentration/content of triacylglycerols, phospholipids, cholesterol, malondialdehyde, glucose and glycogen in the serum and tissues, serum corticosterone and insulin, weights of selected organs, periovarial and epididymal fat and body weight were recordered. Melatonin was not administered to control groups. Oral glucose tolerance tests were carried out before melatonin administration and 9 weeks thereafter.Male and female rats aged 5 weeks were adapted to standard vivarium conditions and artificial light regimen L:D- 12:12 h. The animals were fed MP diet containing 2.5 % fat at least and drank tap water or melatonin solution ad libitum. The rats were weighed twice a week and food and water intake was recorded. After 10 weeks they were sacrificed, organs and tissues were weighed and the aforementioned metabolic parameters were determined in the serum, liver, heart muscle and bone marrow (femur).Melatonin administration decreased significantly the serum triacylglycerol concentration and liver glycogen content in male rats, and it increased liver the phospholipid content in females. Melatonin did not acutely modify the values of other metabolic parameters.Prolonged melatonin administration significantly increased the weight of heart muscle and periovarial fat in females, in males significantly reduced the weight of adrenals, liver, heart muscle and epididymal fat as well as their body weight from day 19 to the end of experiment. Body weight in MEL-drinking females was similar to that in controls. Water and food intake in MEL-drinking males did not differ from controls, in females was temporarily increased in week 6. Prolonged melatonin administration did not significantly influence the glycemia level. The oral glucose tolerance test curves were normal before melatonin administration; one abnormal curve (out of 9 individuals), in the female group and 2 abnormal curves in the male group (out of 9 individuals) were recorded 9 weeks after melatonin administration. Prolonged melatonin treatment resulted in sexual differences in body weight, epididymal and periovarial fat, and heart muscle weight.