Novel human BTB/POZ domain-containing zinc finger protein ZBTB1 inhibits transcriptional activities of CRE
Novel human BTB/POZ domain-containing zinc finger protein ZBTB1 inhibits transcriptional activities of CRE
复制标题
新型含 BTB/POZ 结构域的锌指蛋白 ZBTB1 抑制 CRE 的转录活性
DOI：
10.1007/s11010-011-0911-5
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发表时间：
2011-11-01
影响因子：
4.3
通讯作者：
Wang, Jiu-Cun
中科院分区：
文献类型：
--
作者：
Liu, Qingmei;Yao, Feng;Wang, Jiu-Cun
BTB/POZ蛋白家族通过调节下游基因的转录活性，在许多生物学过程中发挥着关键作用。本文对C2 H2型锌指基因的成员ZBTB 1进行了鉴定。ZBTB 1基因全长2142 bp，编码713个氨基酸，N端有一个与其它已知转录调控因子相同的BTB/POZ结构域，C端有8个典型的锌指C2 H2基序。亚细胞定位分析表明，ZBTB 1定位于细胞核，形成点状结构。转录活性测定表明ZBTB 1是一个转录抑制因子，ZBTB 1在COS 7细胞中的过表达降低了cAMP反应元件（CRE）的转录活性。进一步的研究表明，ZBTB 1的BTB结构域和ZNF基序可能都参与了这种抑制作用。这些结果表明，ZBTB 1蛋白可能作为一个转录抑制因子激活CREB和cAMP介导的信号转导途径，介导细胞功能。
BTB/POZ protein family plays a key role in many biological processes by regulating the transcriptional activities of some downstream genes. Here, we characterized the member of C2H2type zinc finger gene, Zinc finger and BTB domain containing 1 (ZBTB1). The complete sequence of ZBTB1 cDNA contains a 2142 bp open reading frame (ORF) and encodes a 713 amino acid protein with an N-terminal BTB/POZ domain that is similar to the same domain of other known transcription regulators and eight classical zinc finger C2H2motifs in the C-terminus. Subcellular localization analysis demonstrated that ZBTB1 was localized to the nucleus, forming dot-like structures. Transcriptional activity assays showed that ZBTB1 was a transcription repressor and overexpression of ZBTB1 in the COS7 cells reduced the transcriptional activities of cAMP response element (CRE). Further studies showed that the BTB domain and ZNF motifs of ZBTB1 may both be involved in this suppression. These results suggest that ZBTB1 protein may act as a transcription repressor in the activation of CREB and cAMP-mediated signal transduction pathways to mediate cellular functions.