p38 mitogen-activated protein kinase independent SB203580 block of H2O2-induced increase in GABAergic mIPSC amplitude.
p38 mitogen-activated protein kinase independent SB203580 block of H2O2-induced increase in GABAergic mIPSC amplitude.
复制标题
p38 丝裂原激活蛋白激酶独立的 SB203580 阻断 H2O2 诱导的 GABA 能 mIPSC 振幅增加。
DOI：
10.1097/wnr.0b013e3281a032a3
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发表时间：
2007
期刊：
影响因子：
1.7
通讯作者：
Yang,Jay
中科院分区：
文献类型：
--
作者：
Takahashi,Ayako;Mikami,Maya;Yang,Jay
Hydrogen peroxide may serve as a diffusible intermediary messenger under physiological and pathophysiological conditions. H 2 O 2 (1 mM) increased the amplitude of GABAergic miniature inhibitory postsynaptic currents in substantia gelatinosa of the spinal cord. This effect of H 2 O 2 was reversed by the p38 antagonist SB203580, but not by the extracellular signal-regulated kinase 1/2 antagonist PD98059, or the Jun N-terminal kinase antagonist, SP600125. Western blot analysis, however, demonstrated a maximally activated mitogen-activated protein kinase of the slice preparation with no further increase in the phospho-mitogen activated protein kinase by H 2 O 2. Confocal microscopy documented no occult neuronal phospho-p38 induction not detected by Western blot. We conclude that SB203580 blocks H 2 O 2-induced increase in GABAergic miniature inhibitory postsynaptic amplitude independent of its action on p38 mitogen activated protein kinase.
