Hepatic sinusoidal cells in iron overload. Ultrastructural observations.
Hepatic sinusoidal cells in iron overload. Ultrastructural observations.
复制标题
肝窦细胞铁超载。
DOI：
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发表时间：
1978
期刊：
影响因子：
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通讯作者：
H. Neustein
中科院分区：
文献类型：
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作者：
T. Iancu;L. Lichterman;H. Neustein
研究人员对 18 例纯合性 β 地中海贫血患者的肝活检样本中的肝窦细胞超微结构进行了研究。尽管在一些实质细胞中存在电子致密铁，但在开始输血治疗之前获得的活组织检查中，在正弦细胞中没有看到明显的铁。高输血方案后的活检显示实质细胞和窦状细胞中含铁溶酶体的大小和数量逐渐增加。这项研究证实了输血铁大部分分离在网状内皮细胞中的观点。对染色和未染色切片的检查显示库普弗细胞和内皮细胞具有不同类型的含铁溶酶体，这些溶酶体也与肝细胞中看到的大多数含铁溶酶体不同。所描述的溶酶体的细胞特异性形态特征有助于识别铁过载期间的各种细胞。强调了慢性铁超负荷期间吞噬性正弦细胞的重要性。
The ultrastructure of sinusoidal cells was studied in 18 liver biopsies from patients with homozygous beta-thalassemia. No conspicuous iron was seen in sinusoidal cells from biopsies obtained before transfusion therapy was initiated, although electron-dense iron was present in some parenchymal cells. Biopsies following the high transfusion regime showed progressive increase in the size and number of iron-containing lysosomes in both parenchymal and sinusoidal cells. This study confirms the view that transfusional iron is largely segregated in reticuloendothelial cells. Examination of stained and unstained sections showed the Kupffer cells and endothelial cells had different types of iron-containing lysosome that were also dissimilar from most iron-containing lysosomes seen in hepatocytes. The described cell-specific morphological features of the lysosomes facilitate the identification of various cells during iron overload. The importance of phagocytic sinusoidal cells during chronic iron overload is stressed.