Complete Nucleotide Sequences of blaKPC-4- and blaKPC-5-Harboring IncN and IncX Plasmids from Klebsiella pneumoniae Strains Isolated in New Jersey
Complete Nucleotide Sequences of blaKPC-4- and blaKPC-5-Harboring IncN and IncX Plasmids from Klebsiella pneumoniae Strains Isolated in New Jersey
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DOI：
10.1128/aac.01648-12
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发表时间：
2013-01-01
影响因子：
4.9
通讯作者：
Kreiswirth, Barry N.
中科院分区：
文献类型：
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作者：
Chen, Liang;Chavda, Kalyan D.;Kreiswirth, Barry N.
产碳青霉烯酶（KPC）的肺炎克雷伯菌肠杆菌科已成为主要的医院病原菌。bla（KPC）通常位于Tn4401上，存在于革兰氏阴性菌株中，在不同遗传背景的质粒中发现了两种最常见的变体，bla（KPC-2）和bla（KPC-3）。在这项研究中，我们检测了分别于2005年和2006年从新泽西州一家医院分离的两株肺炎克雷伯菌中分离到的携带bla（KPC-4）和bla（KPC-5）的质粒。IncN质粒pBK31551全长84 kb，内含bla（KPC-4）、bla（TEM-1）、qnrB2、aac(3)-Ib、aph（3’）-I、qacF、qacE Delta 1、sul1和dfrA14，这些基因对β -内酰胺类药物、喹诺酮类药物、氨基糖苷类药物、季铵盐化合物和共新恶唑具有耐药性。pBK31551的保守区与其他IncN质粒相似。令人惊讶的是，对Tn4401序列的分析显示，在istA基因中插入了一个携带IS110和tn6901的大元件（8.3 kb），编码乙草醛酶/博来霉素抗性、酒精脱氢酶和s -甲酰基谷胱甘肽水解酶。质粒pBK31567全长47kb，包含bla（KPC-5）、dfrA5、qacE Delta 1和sul1。pBK31567属于一个新的IncX亚群（IncX5），与其他IncX质粒一样具有高度合成的质粒主干；然而，在核苷酸水平上的序列相似性是不同的。bla（KPC-5）基因位于Tn4401元件上，与波多黎各铜绿假单胞菌P28菌株中描述的bla（KPC-5）遗传环境不同。本研究强调了参与bla（KPC）基因传播的多药耐药质粒的遗传多样性，并强调了Tn4401的移动性和可塑性。比较基因组分析为不同不亲和类群的KPC质粒的进化和传播提供了新的见解。
Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacteriaceae have emerged as major nosocomial pathogens. bla(KPC), commonly located on Tn4401, is found in Gram-negative bacterial strains, with the two most common variants, bla(KPC-2) and bla(KPC-3), identified in plasmids with diverse genetic backgrounds. In this study, we examined bla(KPC-4)- and bla(KPC-5)-bearing plasmids recovered from two K. pneumoniae strains, which were isolated from a single New Jersey hospital in 2005 and 2006, respectively. IncN plasmid pBK31551 is 84 kb in length and harbors bla(KPC-4), bla(TEM-1,) qnrB2, aac(3)-Ib, aph(3')-I, qacF, qacE Delta 1, sul1, and dfrA14, which confer resistance to beta-lactams, quinolones, aminoglycosides, quaternary ammonium compounds, and co-trimoxazole. The conserved regions within pBK31551 are similar to those of other IncN plasmids. Surprisingly, analysis of the Tn4401 sequence revealed a large IS110- and Tn6901-carrying element (8.3 kb) inserted into the istA gene, encoding glyoxalase/bleomycin resistance, alcohol dehydrogenase, and S-formylglutathione hydrolase. Plasmid pBK31567 is 47 kb in length and harbors bla(KPC-5), dfrA5, qacE Delta 1, and sul1. pBK31567 belongs to a novel IncX subgroup (IncX5) and possesses a highly syntenic plasmid backbone like other IncX plasmids; however, sequence similarity at the nucleotide level is divergent. The bla(KPC-5) gene is carried on a Tn4401 element and differs from the genetic environment of bla(KPC-5) described in Pseudomonas aeruginosa strain P28 from Puerto Rico. This study under-scores the genetic diversity of multidrug-resistant plasmids involved in the spread of bla(KPC) genes and highlights the mobility and plasticity of Tn4401. Comparative genomic analysis provides new insights into the evolution and dissemination of KPC plasmids belonging to different incompatibility groups.