Teleost multiple tissue (tmt) opsin:: a candidate photopigment regulating the peripheral clocks of zebrafish?
Teleost multiple tissue (tmt) opsin:: a candidate photopigment regulating the peripheral clocks of zebrafish?
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DOI：
10.1016/s0169-328x(03)00059-7
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发表时间：
2003-04-10
期刊：
影响因子：
--
通讯作者：
Foster, RG
中科院分区：
文献类型：
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作者：
Moutsaki, P;Whitmore, D;Foster, RG
斑马鱼的分离器官和细胞系在时钟基因表达中表现出昼夜节律振荡，可以在24小时的光/暗循环中进行。这种细胞光敏性的机制尚不清楚。我们报道鉴定了一个新的视蛋白家族，tmt-opsin，它具有脊椎动物光色素的基因组结构特征，氨基酸身份相当于已知的光色素视蛋白，以及光色素功能所需的必要残基。值得注意的是，tmt-视蛋白在多种神经和非神经组织中表达，包括表现出光可携带时钟的斑马鱼胚胎细胞系。总的来说，这些数据表明tmt-opsin是斑马鱼外周时钟光调节的一个强有力的候选者。2003 Elsevier Science B.V.版权所有
Isolated organs and cell lines from zebrafish exhibit circadian oscillations in clock gene expression that can be entrained to a 24-h light/dark cycle. The mechanism underlying this cellular photosensitivity is unknown. We report the identification of a novel opsin family, tmt-opsin, that has a genomic structure characteristic of vertebrate photopigments, an amino acid identity equivalent to the known photopigment opsins, and the essential residues required for photopigment function. Significantly, tmt-opsin is expressed in a wide variety of neural and non-neural tissues, including a zebrafish embryonic cell line that exhibits a light entrainable clock. Collectively the data suggest that tmt-opsin is a strong candidate for the photic regulation of zebrafish peripheral clocks. 2003 Elsevier Science B.V. All rights reserved.