Rapid Detection of Exosomal MicroRNAs Using Virus-Mimicking Fusogenic Vesicles
Rapid Detection of Exosomal MicroRNAs Using Virus-Mimicking Fusogenic Vesicles
复制标题
使用模拟病毒融合囊泡快速检测外泌体 MicroRNA
DOI：
10.1002/anie.201901997
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发表时间：
2019-06-24
影响因子：
16.6
通讯作者：
Zhang, Chuan
中科院分区：
文献类型：
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作者：
Gao, Xihui;Li, Sha;Zhang, Chuan
外泌体microRNAs （miRNAs）是临床诊断和疾病治疗监测的重要生物标志物。然而，大多数外泌体miRNA检测方法耗时、费力且昂贵。在此，我们报道了一种模拟病毒的融合性囊泡（Vir-FV），它可以在2小时内快速、高效和高通量地检测外泌体mirna。病毒- fv上的融合蛋白可以特异性靶向外泌体上含唾液酸的受体，诱导病毒- fv与外泌体的有效融合。在囊泡内容物混合后，包裹在Vir-FVs中的分子信标与外泌体中的目标mirna特异性杂交，产生荧光。结合流式细胞术，vir - fv不仅可以检测外泌体mirna，还可以通过检测肿瘤相关的mirna来区分肿瘤外泌体和正常外泌体，为快速有效地检测外泌体mirna用于疾病的诊断和预后预测铺平了道路。
Exosomal microRNAs (miRNAs) are important biomarkers for clinical diagnosis and disease treatment monitoring. However, most approaches for exosomal miRNA detection are time-consuming, laborious, and expensive. Herein, we report a virus-mimicking fusogenic vesicle (Vir-FV) that enables rapid, efficient, and high-throughput detection of exosomal miRNAs within 2h. Fusogenic proteins on Vir-FVs can specifically target the sialic-acid-containing receptors on exosomes, inducing efficient fusion of Vir-FVs and exosomes. Upon vesicle content mixing, the molecular beacons encapsulated in Vir-FVs specifically hybridize with the target miRNAs in the exosomes, generating fluorescence. Combined with flow cytometry, the Vir-FVs can not only detect exosomal miRNAs but also distinguish tumor exosomes from normal exosomes by sensing the tumor-related miRNAs, paving the way towards the rapid and efficient detection of exosomal miRNAs for diagnosis and prognosis prediction of diseases.