Natural variation ofCsSTOP1in tea plant (Camellia sinensis) related to aluminum tolerance
Natural variation ofCsSTOP1in tea plant (Camellia sinensis) related to aluminum tolerance
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茶树CsSTOP 1基因的自然变异与耐铝性的关系
DOI：
10.1007/s11104-018-3746-y
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发表时间：
2018-07
期刊：
影响因子：
4.9
通讯作者：
Hua Zhao;Wei Huang;Yange Zhang;Ziwei Zhang;Yong Li;Che Tang;Jie Huang;D. Ni
中科院分区：
文献类型：
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作者：
Hua Zhao;Wei Huang;Yange Zhang;Ziwei Zhang;Yong Li;Che Tang;Jie Huang;D. Ni
茶树（Camellia sinensis（L.）O. Kuntze）原产于中国，其野生祖先广泛分布于中国西南部。茶树作为铝的积累植物，对铝的耐性很强，叶片中铝的积累量较高。在这里，耐铝转录因子CsSTOP 1的特点，并假定调控多个基因的耐铝性至关重要。STOP 1样蛋白的转录调控是保守的，并赋予了在酸性土壤中生存的能力。此外，在5个拟南芥亚种中发现了一个9 bp的缺失，并且CsSTOP 1 Mkdy-OE拟南芥品系比CsSTOP 1 JM 1-OE品系对铝的耐受性更强，这可能是茶树适应酸性土壤的遗传变异的自然选择。鉴于CsSTOP 1 Mkdy等位基因对铝的耐受性更强，茶树从西南地区的原始中心逐渐扩散，本研究表明CsSTOP 1是一种“适应性”性状，在酸性土壤中根毒铝毒的特定环境下，它增加了茶树的适应性。qPCR结果表明，9-bp的缺失不负责转录活性，但该缺失可能影响转录调控水平。
The tea plant (Camellia sinensis(L.) O. Kuntze) is indigenous to China, where its wild ancestors are broadly distributed in Southwest China. As an aluminum (Al) accumulator, tea plant is very tolerant to Al and accumulates Al at high levelin the leaves. Here an Al tolerant transcription factor ofCsSTOP1was characterized and assumed to regulate multiple genes critical for Al tolerance. The transcriptional regulations by STOP1-like proteins were conserved and conferred the ability to survive in acid soil. Furthermore, a 9-bp deletion was found in five varieties ofassamicasubspecies andCsSTOP1Mkdy-OE Arabidopsis lines showed more tolerant to Al thanCsSTOP1JM1-OE lines, which might be the natural selection of the genetic variation for the tea plant’s adaptation to acidic soil. Given theCsSTOP1Mkdyallele more tolerant to Al and tea plant gradually spreading from the original center of Southwest China, this present study suggests thatCsSTOP1is labelled as an ‘adaptive’ trait that increases tea plant fitness in a particular environmental context of rhizotoxicity Al toxicity in acid soil. The qPCR result suggests the 9-bp deletion is not responsible for transcriptional activity while this deletion may affect the transcriptional regulation level.