Human β-defensins and toll-like receptors in the upper airway
Human β-defensins and toll-like receptors in the upper airway
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DOI：
10.1034/j.1398-9995.2003.00180.x
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发表时间：
2003-08-01
期刊：
影响因子：
12.4
通讯作者：
Bachert, C
中科院分区：
文献类型：
--
作者：
Claeys, S;de Belder, T;Bachert, C
背景资料：检测鼻粘膜、扁桃体和腺样体中的先天性免疫标志物可能会对先天性免疫在上呼吸道中的作用产生新的认识。在这项研究中，研究了人β-防御素（HBD）2和3以及Toll样受体（TLR）2和4在各种上呼吸道疾病中的表达。方法：扁桃体疾病（n = 18）、肥大性腺样体（n = 10）和鼻窦疾病（n = 30）患者的手术样本应用免疫组化方法检测慢性鼻窦炎、鼻息肉、鼻甲黏膜组织的免疫组织化学变化。采用实时荧光定量PCR（real-time polymerase chain reaction，PCR）方法检测扁桃体组织中HBD-2、3 mRNA和TLR-2、4 mRNA的表达。HBD-2和HBD-3 mRNA的定量显示，扁桃体组织中的表达比腺样体中的表达高10倍以上，而在鼻活检中，只能测量到可忽略的防御素表达。TLR-4的各种组织之间没有显着差异，而TLR-2在腺样体中的表达显着较低，与其他tissues.Conclusion：这些结果表明，较强的防御素在扁桃体组织中的表达鼻和鼻旁粘膜和腺样体。Toll样受体在所有这些组织中的表达说明了上呼吸道粘膜可能具有重要的免疫前哨功能。
Background: Measurement of innate markers in nasal mucosa, tonsils and adenoids might lead to new views about the role of innate immunity in the upper airway. In this study, the expression of human beta-defensins (HBD) 2 and 3 and toll-like receptors (TLR) 2 and 4 in various upper airway diseases was investigated.Methods: Surgical samples from patients with tonsillar disease (n = 18), hypertrophic adenoids (n = 10) and sinonasal disease (n = 30) (chronic sinusitis, nasal polyps, turbinate mucosa as controls) were investigated by immunohistochemistry. Quantification of HBD-2 and 3 mRNA, TLR-2 and 4 mRNA expression was performed by real-time polymerase chain reaction (PCR).Results: Immunohistochemistry revealed a strong expression of HBD-2 in tonsillar tissue. Quantification of HBD-2 and HBD-3 mRNA showed a more than tenfold higher expression in tonsillar tissue than in adenoids, whereas in nasal biopsies, only negligible defensin expression could be measured. No significant differences were found for TLR-4 between the various tissues, whereas TLR-2 expression in adenoids was significantly lower compared with other tissues.Conclusion: These results demonstrate a strong defensin expression in tonsillar tissue compared with nasal and paranasal mucosa and adenoids. Toll-like receptor expression in all these tissues illustrates a possibly important immunological sentinel function of upper airway mucosa.