Shifting the limits in wheat research and breeding using a fully annotated reference genome
Shifting the limits in wheat research and breeding using a fully annotated reference genome
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DOI：
10.1126/science.aar7191
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发表时间：
2018-08-17
期刊：
影响因子：
56.9
通讯作者：
Uauy, Cristobal
中科院分区：
文献类型：
--
作者：
Appels, Rudi;Eversole, Kellye;Uauy, Cristobal
一个注释的参考序列，代表六倍体面包小麦基因组中的21个假分子进行了分析，以确定分布和基因组背景的编码和非编码元件在A，B，和D亚基因组。估计覆盖了94%的基因组，包含107，891个高置信度的基因模型，该组装通过提供代表小麦发育主要阶段的转录组图谱，使组织和发育阶段相关的共表达网络的发现成为可能。动态复杂的基因家族参与环境适应和最终使用的质量，揭示了在亚基因组分辨率和上下文已知的农艺单基因或数量性状位点。这一社区资源通过提高对小麦生物学和基因组辅助育种的理解，为加速小麦研究和应用奠定了基础。
An annotated reference sequence representing the hexaploid bread wheat genome in 21 pseudomolecules has been analyzed to identify the distribution and genomic context of coding and noncoding elements across the A, B, and D subgenomes. With an estimated coverage of 94% of the genome and containing 107,891 high-confidence gene models, this assembly enabled the discovery of tissue-and developmental stage-related coexpression networks by providing a transcriptome atlas representing major stages of wheat development. Dynamics of complex gene families involved in environmental adaptation and end-use quality were revealed at subgenome resolution and contextualized to known agronomic single-gene or quantitative trait loci. This community resource establishes the foundation for accelerating wheat research and application through improved understanding of wheat biology and genomics-assisted breeding.