Angiogenesis and myogenesis in mouse tibialis anterior muscles during distraction osteogenesis: VEGF, its receptors, and myogenin genes expression
Angiogenesis and myogenesis in mouse tibialis anterior muscles during distraction osteogenesis: VEGF, its receptors, and myogenin genes expression
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DOI：
10.1002/jor.22136
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发表时间：
2012-11-01
影响因子：
2.8
通讯作者：
Yasui, Natsuo
中科院分区：
文献类型：
--
作者：
Nishisho, Toshihiko;Yukata, Kiminori;Yasui, Natsuo
牵张成骨后周围骨骼肌中发生血管生成和肌生成，但其分子机制尚不清楚。本研究观察了肌肉延长后的形态学特征及其受体血管内皮生长因子（VEGF）的时程变化（VEGFR-1和VEGFR-2）和肌细胞生成素基因表达谱与胫骨前肌（TA）肌肉中的血管生成和肌细胞生成相关，采用牵张成骨小鼠模型，其中包括1周的等待期（潜伏期）、2周间歇性牵引（牵引期）和5周重塑期（巩固期）。肉眼观察结果显示，与术前相比，在手术结束时，延长的TA肌肉增加至约42%长，重10%。在牵张阶段，血管内皮细胞、肌细胞生成素阳性卫星细胞和肌细胞中的VEGF及其受体被诱导，随后，毛细血管进展和肌生成增加。实时定量RT-PCR结果显示，在肌肉延长过程中，VEGF、Vegfr-1、Vegfr-2和myogenin基因表达增强。VEGF和Vegfr-1在巩固期血管生成衰退后上调。我们的结论是，上调VEGF及其受体的机械张力应力可能参与的血管生成和肌肉生长的过程中延长的肌肉。(c)2012年骨科研究学会。出版社：Wiley Periodicals，Inc.骨科研究杂志30：17671773，2012年
Angiogenesis and myogenesis occur in the surrounding skeletal muscles following distraction osteogenesis, but their molecular mechanisms remain unclear. The present study investigated morphological features of lengthened muscles and the time course change of vascular endothelial growth factor (VEGF), its receptors (VEGFR-1 and VEGFR-2) and myogenin gene expression profiles related to angiogenesis and myogenesis in tibialis anterior (TA) muscles with a mouse model of distraction osteogenesis, which involves 1 week of waiting period (latency phase), 2 weeks of intermittent distraction (distraction phase), and 5 weeks of remodeling period (consolidation phase). Macroscopic findings showed that lengthened TA muscles increased to approximately 42% longer and 10% heavier at the end of the process when compared to pre-surgery. During the distraction phase, VEGF and its receptors were induced in the vascular endothelial cells, myogenin-positive satellite cells and myocytes, and subsequently, capillary progression and myogenesis were increased. Real-time RT-PCR showed that Vegf, Vegfr-1, Vegfr-2, and myogenin genes expression was enhanced during the muscle lengthening. Vegf and Vegfr-1 were upregulated following the recession of angiogenesis at the consolidation phase. We conclude that upregulation of VEGF and its receptors by mechanical tension-stress could be involved in the process of angiogenesis and myogenesis in lengthened muscles. (c) 2012 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 30:17671773, 2012