ETHANOL DIFFERENTIALLY REGULATES G-PROTEINS IN NEURAL CELLS
ETHANOL DIFFERENTIALLY REGULATES G-PROTEINS IN NEURAL CELLS
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DOI：
10.1016/s0006-291x(88)81060-x
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发表时间：
1988-08-30
影响因子：
3.1
通讯作者：
HENTELEFF, M
中科院分区：
文献类型：
--
作者：
CHARNESS, ME;QUERIMIT, LA;HENTELEFF, M
克隆细胞系和神经细胞系与乙醇的长期孵育可不同程度地减少激素和霍乱毒素对 cAMP 积累的刺激。在 NG108-15 神经母细胞瘤 x 神经胶质瘤杂交细胞系中，这种异源脱敏与 GS α 表达减少 42% 相关。 G1 .cntdot 没有显着变化。相比之下，亲本神经母细胞瘤细胞系N18TG2的乙醇处理几乎没有引起对激素或霍乱毒素的反应损失，并且GS α没有显着变化。或 G1 .cntdot。 .α..乙醇诱导N1E-115神经母细胞瘤细胞异源脱敏；然而，该细胞系显示 G1 .cntdot 剂量依赖性增加。 。阿尔法。后来 G5 .cntdot 减少。因此，乙醇通过不同机制在相关神经细胞系中引起激素刺激的cAMP积累的异源脱敏。
Long-term incubation of clonal and neural cell lines with ethanol differentially reduces the stimulation of cAMP accumulation by hormones and cholera toxin. In the NG108-15 neuroblastoma x glioma hybrid cell line, this heterologous desensitization was associated with a 42% reduction in the expression of GS .alpha. and no significant change in G1 .cntdot. .alpha.. By contrast, ethanol treatment of the parental neuroblastoma cell line N18TG2 caused little loss of response to hormones or cholera toxin and no significant change in GS .alpha. or G1 .cntdot. .alpha.. Ethanol induced heterologous desensitization in N1E-115 neuroblastoma cells; however, this cell line showed a dose-dependent increase in G1 .cntdot. .alpha. and a later decrease in G5 .cntdot. .alpha.. Thus, ethanol causes heterologous desensitization of hormone-stimulated cAMP accumulation by different mechanisms in related neural cell lines.