INACTIVATION OF RESPIRATORY SYNCYTIAL VIRUS BY DETERGENTS AND DISINFECTANTS
INACTIVATION OF RESPIRATORY SYNCYTIAL VIRUS BY DETERGENTS AND DISINFECTANTS
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DOI：
10.1097/00006454-199307000-00007
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发表时间：
1993-07-01
影响因子：
3.6
通讯作者：
HARKNESS, SH
中科院分区：
文献类型：
--
作者：
KRILOV, LR;HARKNESS, SH
在体外试验系统中评价了多种洗涤剂和消毒剂对呼吸道合胞病毒（RSV）的活性。将等体积的RSV和测试试剂的连续10倍稀释液在4 ℃下混合5分钟。将每种混合物中的RSV滴度与单独未处理的RSV的滴度进行比较。在输入RSV滴度范围为2.6 x 10（3）至2 x 10（7）空斑形成单位/ml的14项实验中，5.25%次氯酸钠（通用漂白剂）的10倍稀释液灭活了病毒（滴度下降大于或等于3个对数）。在较低RSV滴度的情况下，在漂白剂的100倍稀释度下也观察到灭活。胎牛血清浓度高达50%的有机负荷没有减少漂白效果。还定义了来苏（R）、聚维酮碘、Amphyl（R）、Hibiclens（R）、Osyl（R）、乙醇和Listermint（R）的RSV灭活程度。接触时间短，结果的可重现性以及随着有机负荷增加的持续有效性都表明清洁剂和消毒剂在减少RSV感染传播方面可能发挥重要作用。
The activity of a number of detergents and disinfectants against respiratory syncytial virus (RSV) was evaluated in an in vitro assay system. Equal volumes of RSV and serial 10-fold dilutions of the test agents were mixed at 4-degrees-C for 5 minutes. The RSV titer in each mixture was compared with that of untreated RSV alone. In 14 experiments with input RSV titers ranging from 2.6 x 10(3) to 2 x 10(7) plaque-forming units/ml, a 10-fold dilution of 5.25% sodium hypochlorite (generic bleach) inactivated (greater-than-or-equal-to 3-log reduction in titer) the virus. With lower RSV titers inactivation was also observed at a 100-fold dilution of bleach. Fetal calf serum concentrations up to 50% as an organic load did not diminish the bleach effect. The degree of RSV inactivation was also defined for Lysol(R), povidone-iodine, Amphyl(R), Hibiclens(R), Osyl(R), ethanol and Listermint(R). The short contact time, the reproducible nature of the findings and the continued effectiveness with increasing organic loads all suggest that detergents and disinfectants can potentially play an important role in decreasing the spread of RSV infection.