Influence of temperature on the composition and activity of denitrifying soil communities
Influence of temperature on the composition and activity of denitrifying soil communities
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DOI：
10.1111/j.1574-6941.2010.00884.x
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发表时间：
2010-07-01
影响因子：
4.2
通讯作者：
Conrad, Ralf
中科院分区：
文献类型：
--
作者：
Braker, Gesche;Schwarz, Julia;Conrad, Ralf
通过室内对照试验，研究了温度对农业土壤中反硝化菌活性、大小及群落组成的影响。土壤浆液在不同温度（4、15、20、25和37℃）下在无限制基质条件下孵育3周。利用最可能数（MPN）技术确定了一般硝酸盐还原剂群落的丰度；通过测定潜在的反硝化酶活性、末端限制性片段长度多态性以及亚硝酸盐还原酶基因扩增子（nirK和nirS）的系统发育分析来评估反硝化菌活性和群落组成。随着孵育温度的升高，反硝化活性逐渐增强，而且硝酸盐还原剂丰度增加，反硝化菌群落组成也有所不同。从最高稀释度的MPN系列中纯化的分离株的遗传和生理特征强调了群落差异。总体而言，温度不仅明显影响过程速率，而且导致反硝化菌的富集和群落组成的变化。
The impacts of temperature on the activity and on the size as well as on the community composition of denitrifiers in an agricultural soil were studied in a controlled laboratory experiment. Soil slurries were incubated at different temperatures (4, 15, 20, 25, and 37 degrees C) under nonlimiting substrate conditions for 3 weeks. The abundance of the nitrate-reducer community in general was determined using the most probable number (MPN) technique; denitrifier activity and community composition were assessed by measuring potential denitrifier enzyme activity and by terminal restriction fragment length polymorphisms as well as by phylogenetic analysis of nitrite reductase gene amplicons (nirK and nirS). Increasing incubation temperatures resulted in gradually enhanced denitrification activity, but also in higher abundance of nitrate reducers and in different denitrifier community compositions. Genetic and physiological characterization of isolates purified from the highest dilution of the MPN series emphasized community differences. Overall, temperature apparently not only affected process rates but also resulted in the enrichment of denitrifiers and shifts in the community composition.