Glycosylation might provide endothelial zip codes for organ-specific leukocyte traffic into inflammatory sites
Glycosylation might provide endothelial zip codes for organ-specific leukocyte traffic into inflammatory sites
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DOI：
10.1016/s0002-9440(10)64210-1
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发表时间：
2002-08-01
影响因子：
6
通讯作者：
Renkonen, R
中科院分区：
文献类型：
--
作者：
Renkonen, J;Tynninen, O;Renkonen, R
炎性疾病的特征在于白细胞浸润到组织中。淋巴细胞上的L-选择素及其内皮糖基化配体在淋巴细胞外渗的启动中起作用。对400多例甲状腺炎、心肌炎、银屑病、血管炎、溃疡性结肠炎及其相应的非炎症组织标本进行了免疫组织化学染色，这些标本均使用了抗功能活性聚糖修饰的L-选择素配体（即唾液酸化Lewis x（sLex、2F 3和HECA-452）或硫酸化延伸核心1乳糖胺（MECA-79））的单克隆抗体。在对照组织中，毛细血管后小静脉中sLex或磺基sLex聚糖的内皮表达不存在或较低。在所有发炎组织中检测到sLex或磺基sLex聚糖的内皮表达的从头诱导。此外，每个器官都携带自己的sLex或磺基sLex聚糖修饰，即邮政编码。我们的结果表明，这些邮政编码聚糖可能为器官选择性白细胞运输提供了手段，可用于选择性白细胞运输抑制。
Inflammatory diseases are characterized by the leukocyte infiltration into tissues. L-selectin on lymphocytes and its endothelial glycosylated ligands are instrumental in the initiation of lymphocyte extravasation. Immunohistochemical stainings with monoclonal antibodies against functionally active glycan-decorated L-selectin ligands, ie, sialyl-Lewis x (sLex, 2F3, and HECA-452) or sulfated extended core 1 lactosamine (MECA-79), were performed on more than 400 specimen representatives for thyroiditis, myocarditis, psoriasis, vasculitis, ulcerative colitis, and their corresponding noninflamed tissues. The endothelial expression of sLex or sulfo sLex glycans in postcapillary venules was either absent or low in control tissues. The de novo induction of endothelial expression of sLex or sulfo sLex glycans was detected in all inflamed tissues. Furthermore, each organ carried its own modification of sLex or sulfo sLex glycans, ie, zip code. Our results suggest that these zip code glycans may provide means for organ selective leukocyte traffic that could be used in selective leukocyte traffic inhibition.