Effects of a peracetic acid disinfection protocol on the biocompatibility and biomechanical properties of human patellar tendon allografts
Effects of a peracetic acid disinfection protocol on the biocompatibility and biomechanical properties of human patellar tendon allografts
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DOI：
10.1023/b:catb.0000046071.11459.bd
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发表时间：
2004-01-01
影响因子：
1.5
通讯作者：
Kearney, J. N.
中科院分区：
文献类型：
--
作者：
Lomas, R. J.;Jennings, L. M.;Kearney, J. N.
从人类尸体捐赠者身上获取的同种异体髌腱移植物被广泛用于替代受损的十字韧带。与源自尸体捐赠者的其他同种异体组织移植一样，人们担心疾病从捐赠者传播到接受者的可能性。此外，回收和后续处理方案使移植物面临环境污染的风险。由于这些原因，这些移植物在临床使用之前必须进行消毒或灭菌。已经开发出一种利用过乙酸 (PAA) 的高水平消毒方案，并研究了其对同种异体髌腱生物相容性和生物力学的影响。根据体外评估，PAA 消毒不会使移植物具有细胞毒性或容易引起炎症反应。然而，该方案被证明可以增加基质中肌腱纤维之间的间隙尺寸，并使移植物更容易被胶原酶消化。肌腱的生物力学研究表明，PAA 处理对肌腱的极限拉伸应力或杨氏模量没有影响，并且 PAA 处理的肌腱的极限应变明显更高。
Patellar tendon allografts, retrieved from cadaveric human donors, are widely used for replacement of damaged cruciate ligaments. In common with other tissue allografts originating from cadaveric donors, there are concerns regarding the potential for disease transmission from the donor to the recipient. Additionally, retrieval and subsequent processing protocols expose the graft to the risk of environmental contamination. For these reasons, disinfection or sterilisation protocols are necessary for these grafts before they are used clinically. A high-level disinfection protocol, utilising peracetic acid (PAA), has been developed and investigated for its effects on the biocompatibility and biomechanics of the patellar tendon allografts. PAA disinfection did not render the grafts either cytotoxic or liable to provoke an inflammatory response as assessed in vitro. However, the protocol was shown to increase the size of gaps between the tendon fibres in the matrix and render the grafts more susceptible to digestion with collagenase. Biomechanical studies of the tendons showed that PAA treatment had no effect on the ultimate tensile stress or Young's modulus of the tendons, and that ultimate strain was significantly higher in PAA treated tendons.