Experimental estimation of blood flow velocity through simulation of intravital microscopic imaging in micro-vessels by different image processing methods
Experimental estimation of blood flow velocity through simulation of intravital microscopic imaging in micro-vessels by different image processing methods
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DOI：
10.1016/j.mvr.2010.07.007
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发表时间：
2010-12-01
影响因子：
3.1
通讯作者：
Lin, Kang-Ping
中科院分区：
文献类型：
--
作者：
Huang, Tzung-Chi;Lin, Wen-Chen;Lin, Kang-Ping
微血管中红细胞运动速度的定量分析是动态观察微血管机制的技术之一。微血管中红细胞的流量测量一直是一个难题。使用逐帧分析的用于速度测量的图像处理是常见的方法。计算的准确性，这是算法依赖，很少被审查。在本文中，我们评估现有的方法，其中包括互相关法，霍夫变换法，光流法，通过应用这些方法模拟微血管图像序列的准确性。在各种微血管与随机红细胞运动的模拟实验中应用的评价。在模拟中考虑了相同微血管中不同红细胞密度和速度下的血流变化。并分别考察了不同流型和不同血管形状下的计算精度。在此基础上，提出了一种上级互相关法和Hough变换法的光流法测量红细胞速度的方法。研究表明，光流法适用于精确测量红细胞在大小微血管中的运动速度。(C)2010年爱思唯尔公司All rights reserved.
Quantization of red blood cell (RBC) velocity in micro-vessel is one of the techniques for dynamic observation of microvascular mechanisms. The flow measurement of RBC in micro-vessels is still a challenge nowadays. Image processing for velocity measurement using a frame by frame analysis is a common approach. The accuracy of the calculations, which is algorithm dependant, has rarely been examined. In this paper, we evaluated the accuracy of the existing methods, which includes cross correlation method, Hough transform method, and optical flow method, by applying these methods to simulated micro-vessel image sequences. Simulated experiments in various micro-vessels with random RBC motion were applied in the evaluation. The blood flow variation in the same micro-vessels with different RBC densities and velocities was considered in the simulations. The calculation accuracy of different flow patterns and vessel shapes were also examined, respectively. Based on the comparison, the use of an optical flow method, which is superior to a cross-correlation method or a Hough transform method, is proposed for measuring RBC velocity. The study indicated that the optical flow method is suitable for accurately measuring the velocity of the RBCs in small or large micro-vessels. (C) 2010 Elsevier Inc. All rights reserved.