Molecular characterization of prosomeric and intraprosomeric subdivisions of the embryonic zebrafish diencephalon
Molecular characterization of prosomeric and intraprosomeric subdivisions of the embryonic zebrafish diencephalon
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DOI：
10.1002/cne.23221
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发表时间：
2013-04-01
影响因子：
2.5
通讯作者：
Hauptmann, Giselbert
中科院分区：
文献类型：
--
作者：
Lauter, Gilbert;Soll, Iris;Hauptmann, Giselbert
在早期神经管发育过程中，由信号梯度提供的位置信息被转化为横向和纵向转录因子表达区域的网格。在这个网格内定义不同组织发生区域的转录因子特异性编码在脊椎动物中是进化保守的，可能反映了一种潜在的脊椎动物基本构造模式。与四足动物丰富的比较基因表达研究相比，硬骨鱼的比较数据要少得多。我们使用灵敏的多色荧光原位杂交技术绘制了斑马鱼胚胎间脑调节基因表达区域的详细图谱。这种技术的高分辨率使我们能够分辨不同转录本相邻和重叠的基因表达。我们发现斑马鱼基因表达模式的相对地形与四足动物的同源基因高度相似，并且与间脑翼板和基板的三个前脑节组织一致。我们的分析进一步证明了前脑节1、2和3（p1、p2和p3）内前脑节细分的保守性。我们确定了斑马鱼p1的三分结构，让人联想到四足动物的连合前（PcP）、连合旁（JcP）和连合（CoP）顶盖前区。构建的详细间脑转录因子基因表达图谱进一步确定了斑马鱼在分子上不同的丘脑和丘脑前的头端和尾端区域以及一个丘脑前隆起组织发生区域。我们的比较基因表达分析符合从鱼类到哺乳动物的无羊膜动物和有羊膜动物脊椎动物间脑具有共同基本构造模式的观点。《比较神经学杂志》2013年521卷：1093 - 1118页。（c）2012威利期刊公司
During development of the early neural tube, positional information provided by signaling gradients is translated into a grid of transverse and longitudinal transcription factor expression domains. Transcription factor specification codes defining distinct histogenetic domains within this grid are evolutionarily conserved across vertebrates and may reflect an underlying common vertebrate bauplan. When compared to the rich body of comparative gene expression studies of tetrapods, there is considerably less comparative data available for teleost fish. We used sensitive multicolor fluorescent in situ hybridization to generate a detailed map of regulatory gene expression domains in the embryonic zebrafish diencephalon. The high resolution of this technique allowed us to resolve abutting and overlapping gene expression of different transcripts. We found that the relative topography of gene expression patterns in zebrafish was highly similar to those of orthologous genes in tetrapods and consistent with a three-prosomere organization of the alar and basal diencephalon. Our analysis further demonstrated a conservation of intraprosomeric subdivisions within prosomeres 1, 2, and 3 (p1, p2, and p3). A tripartition of zebrafish p1 was identified reminiscent of precommissural (PcP), juxtacommissural (JcP), and commissural (CoP) pretectal domains of tetrapods. The constructed detailed diencephalic transcription factor gene expression map further identified molecularly distinct thalamic and prethalamic rostral and caudal domains and a prethalamic eminence histogenetic domain in zebrafish. Our comparative gene expression analysis conformed with the idea of a common bauplan for the diencephalon of anamniote and amniote vertebrates from fish to mammals. J. Comp. Neurol. 521:10931118, 2013. (c) 2012 Wiley Periodicals, Inc.