Estimation of the Turnover of 3‐Methoxytyramine in the Rat Striatum by HPLC with Electrochemical Detection: Implications for the Sequence in the Cerebral Metabolism of Dopamine
Estimation of the Turnover of 3‐Methoxytyramine in the Rat Striatum by HPLC with Electrochemical Detection: Implications for the Sequence in the Cerebral Metabolism of Dopamine
复制标题
通过电化学检测 HPLC 估算大鼠纹状体中 3-甲氧基酪胺的周转率：对多巴胺脑代谢序列的影响
DOI：
10.1111/j.1471-4159.1982.tb08634.x
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发表时间：
1982
影响因子：
4.7
通讯作者：
S. J. Spaan
中科院分区：
文献类型：
--
作者：
B. Westerink;S. J. Spaan
摘要：建立了一种测定神经组织中3‐甲氧基酪胺（3‐MT）的高灵敏度方法。该方法基于在Sephadex g10小柱上快速分离3 - MT，然后采用反相高效液相色谱法与旋转圆盘电化学检测器相结合。该方法的检出限（0.5-1 pmol/组织样品）约为微波杀灭大鼠对照值的10%。3‐MT和多巴胺可以在同一层析运行中定量。邻苯二酮抑制儿茶酚- O -甲基转移酶导致3 - MT呈指数下降。根据这一指数下降，计算出3 - MT的周转率为1.9 nmol/g/h。同一组大鼠同香草酸周转率为9.1 nmol/g/h。从这些数据可以得出结论，在大鼠纹状体中，大约80%的高香草酸是由3,4‐二羟基苯基乙酸形成的，20%是由3‐MT形成的。
Abstract: A highly sensitive method for the determination of 3‐methoxytyramine (3‐MT) in nervous tissue is described. The method is based on a rapidly performed isolation of 3‐MT on small columns of Sephadex G 10, followed by reverse‐phase high‐performance liquid chromatography in conjunction with a rotating disk electrochemical detector. The detection limit of the assay (0.5–1 pmol/tissue sample) is about 10% of control value for microwave‐killed rats. 3‐MT as well as dopamine could be quantified in the same chromatographic run. Inhibition of catechol‐O‐methyl transferase with tropolone resulted in an exponential decline of 3‐MT. From this exponential decline a turnover rate for 3‐MT of 1.9 nmol/g/h was calculated. In the same group of rats the turnover rate of homovanillic acid was 9.1 nmol/g/h. From these data it is concluded that in the rat striatum about 80% of homovanillic acid is formed from 3,4‐dihydroxyphenylacetic acid and 20% from 3‐MT.