Spatial H2O2 signaling specificity: H2O2 from chloroplasts and peroxisomes modulates the plant transcriptome differentially.
Spatial H2O2 signaling specificity: H2O2 from chloroplasts and peroxisomes modulates the plant transcriptome differentially.
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DOI：
10.1093/mp/ssu070
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发表时间：
2014-07
期刊：
影响因子：
27.5
通讯作者：
N. Sewelam;Nils Jaspert;Katrien Van der Kelen;V. Tognetti;Jessica Schmitz;Henning Frerigmann;E. Stahl
中科院分区：
文献类型：
--
作者：
N. Sewelam;Nils Jaspert;Katrien Van der Kelen;V. Tognetti;Jessica Schmitz;Henning Frerigmann;E. Stahl
过氧化氢(H_2O_2)在真核生物中作为一种信号分子发挥作用，但其信号功能的特异性在很大程度上仍未被揭示。在这里，我们分析了来自两个不同植物细胞室的适度产生的过氧化氢是否对植物转录组有不同的影响。拟南芥在叶绿体中高表达乙醇酸氧化酶(Fahnenstich等人，2008；Balazadeh等人，2012)和缺乏过氧化氢酶的植物(Quval等人，2007；Inzéet al.，2012)在非光呼吸条件下生长，然后转移到光呼吸条件下，以促进两个细胞器中过氧化氢的产生。我们发现，起源于特定细胞器的H_2O_2诱导了两种类型的反应：一种是独立于H_2O_2产生的亚细胞部位整合信号，另一种是依赖于H_2O_2产生部位。过氧化体中产生的过氧化氢诱导参与蛋白质修复反应的转录本，而叶绿体中产生的过氧化氢诱导早期信号反应，包括参与产生二级信号信使的转录因子和生物合成基因。有一种明显的偏向于诱导参与对氯塑料产生的过氧化氢伤害和病原体攻击的反应的基因，包括吲哚硫代葡萄糖苷、茶碱蛋白和豆甾醇生物合成基因。这些转录反应伴随着4-甲氧基-吲哚-3-甲基硫代葡萄糖苷和豆甾醇的积累。
Hydrogen peroxide (H2O2) operates as a signaling molecule in eukaryotes, but the specificity of its signaling capacities remains largely unrevealed. Here, we analyzed whether a moderate production of H2O2 from two different plant cellular compartments has divergent effects on the plant transcriptome. Arabidopsis thaliana overexpressing glycolate oxidase in the chloroplast (Fahnenstich et al., 2008; Balazadeh et al., 2012) and plants deficient in peroxisomal catalase (Queval et al., 2007; Inzé et al., 2012) were grown under non-photorespiratory conditions and then transferred to photorespiratory conditions to foster the production of H2O2 in both organelles. We show that H2O2 originating in a specific organelle induces two types of responses: one that integrates signals independently from the subcellular site of H2O2 production and another that is dependent on the H2O2 production site. H2O2 produced in peroxisomes induces transcripts involved in protein repair responses, while H2O2 produced in chloroplasts induces early signaling responses, including transcription factors and biosynthetic genes involved in production of secondary signaling messengers. There is a significant bias towards the induction of genes involved in responses to wounding and pathogen attack by chloroplastic-produced H2O2, including indolic glucosinolates-, camalexin-, and stigmasterol-biosynthetic genes. These transcriptional responses were accompanied by the accumulation of 4-methoxy-indol-3-ylmethyl glucosinolate and stigmasterol.