Stem Cell Plasticity in Muscle and Bone Marrow
Stem Cell Plasticity in Muscle and Bone Marrow
复制标题
肌肉和骨髓中的干细胞可塑性
DOI：
--
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发表时间：
2001
影响因子：
5.2
通讯作者：
K. Hirschi
中科院分区：
文献类型：
--
作者：
M. Goodell;K. Jackson;S. Majka;Tiejuan Mi;Hongyu Wang;J. Pocius;C. Hartley;M. Majesky;M. Entman;L. Michael;K. Hirschi
摘要：最近的发现证明了组织源干细胞具有非凡的可塑性，这引发了关于细胞谱系关系的基本问题，并暗示了基于细胞的新疗法的潜力。我们已经在来自骨骼肌和来自骨髓的干细胞之间的潜在互惠关系中研究了这一现象。我们发现成年小鼠骨骼肌来源的细胞具有显著的造血分化能力。用酶消化法提取肌肉细胞，体外培养5d，分别与可区分的全骨髓细胞一起接种于6个致死剂量照射的受者体内。6周和12周后，所有受者都表现出代表所有主要成人血统的肌肉来源细胞的高水平植入。肌肉细胞后代对外周血的平均总贡献率为56%，表明培养的肌肉细胞产生的造血活性大约是整个骨髓的10-14倍。尽管肌源性造血干细胞的身份尚不清楚，但它们可能与肌肉卫星细胞相同，其中一些缺乏肌源性调节因子，可以对造血信号做出反应。我们还发现，骨髓中的干细胞可以促进心肌缺血损伤后的心肌修复和新生血管。我们将高纯度的骨髓干细胞移植到致死性照射的小鼠体内，这些小鼠随后因冠状动脉闭塞和再灌流而变得缺血。移植的干细胞或其后代分化为心肌细胞和内皮细胞，并有助于形成功能组织。
Abstract: Recent discoveries have demonstrated the extraordinary plasticity of tissue‐derived stem cells, raising fundamental questions about cell lineage relationships and suggesting the potential for novel cell‐based therapies. We have examined this phenomenon in a potential reciprocal relationship between stem cells derived from the skeletal muscle and from the bone marrow. We have discovered that cells derived from the skeletal muscle of adult mice contain a remarkable capacity for hematopoietic differentiation. Cells prepared from muscle by enzymatic digestion and 5 day in vitro culture were harvested and introduced into each of six lethally irradiated recipients together with distinguishable whole bone marrow cells. Six and twelve weeks later, all recipients showed high‐level engraftment of muscle‐derived cells representing all major adult blood lineages. The mean total contribution of muscle cell progeny to peripheral blood was 56%, indicating that the cultured muscle cells generated approximately 10‐ to 14‐fold more hematopoietic activity than whole bone marrow. Although the identity of the muscle‐derived hematopoietic stem cells is still unknown, they may be identical to muscle satellite cells, some of which lack myogenic regulators and could respond to hematopoietic signals. We have also found that stem cells in the bone marrow can contribute to cardiac muscle repair and neovascularization after ischemic injury. We transplanted highly purified bone marrow stem cells into lethally irradiated mice that subsequently were rendered ischemic by coronary artery occlusion and reperfusion. The engrafted stem cells or their progeny differentiated into cardiomyocytes and endothelial cells and contributed to the formation of functional tissue.
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肝脏作为干细胞和谱系系统。
DOI：
10.1152/ajpgi.1992.263.2.g139
发表时间：
1992
期刊：
The American journal of physiology
影响因子：
--
作者：
Sigal,SH;Brill,S;Fiorino,AS;Reid,LM
通讯作者：
Reid,LM
影响因子：
15.9
作者：
Klug, MG;Soonpaa, MH;Field, LJ
通讯作者：
Field, LJ
影响因子：
2.7
作者：
Cornelison, DDW;Wold, BJ
通讯作者：
Wold, BJ
影响因子：
37.8
作者：
Reinecke, H;Zhang, M;Murry, CE
通讯作者：
Murry, CE
DOI：
10.1152/ajpheart.1995.269.6.h2147
发表时间：
1995-12-01
影响因子：
4.8
作者：
Michael, LH;Entman, ML;Ballantyne, CM
通讯作者：
Ballantyne, CM