A reduced genome decreases the host carrying capacity for foreign DNA.
A reduced genome decreases the host carrying capacity for foreign DNA.
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DOI：
10.1186/1475-2859-13-49
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发表时间：
2014-04-01
影响因子：
6.4
通讯作者：
Yomo T
中科院分区：
文献类型：
--
作者：
Akeno Y;Ying BW;Tsuru S;Yomo T
宿主-质粒相互作用主要是根据质粒的影响进行讨论，而宿主基因组变异对宿主与外源DNA相互作用的贡献仍不清楚。最近从常用宿主菌株野生型菌株 MG1655 中产生了一种具有基因组大小减小的所谓“干净基因组”（即 MDS42）的菌株。对这两种大肠杆菌菌株中质粒负荷的影响进行定量评估不仅可以了解减少的基因组如何对外源 DNA 作出反应，还可以深入了解这些菌株的正确应用。对于野生型和清洁基因组菌株来说，由于携带外源 DNA 的成本而导致的生长下降是相似的。在两种菌株中均观察到生长速率与外源 DNA 总量之间呈负相关，但对于具有干净基因组的菌株，发现了更好的理论拟合和更高的统计显着性。与野生型菌株相比，清洁基因组菌株对外源DNA的携带能力降低，这很大程度上归因于其限制外源DNA复制的能力。在具有干净基因组的菌株中观察到将能量和资源分配给基因表达而不是DNA复制的倾向。拥有干净的基因组将质粒拷贝数限制为野生型等效负载。结果表明，野生型菌株对外源DNA具有更大的耐受性，如内共生，并且使用具有干净基因组的菌株将有利于需要精确控制和理论预测的应用。
Host-plasmid interactions have been discussed largely in terms of the influences of plasmids, whereas the contributions of variations in host genomes to host interactions with foreign DNA remain unclear. A strain with a so-called “clean genome” (i.e., MDS42) of reduced genome size has recently been generated from the wild-type strain MG1655, a commonly used host strain. A quantitative evaluation of the influence of plasmid burdens in these two Escherichia coli strains can not only provide an understanding of how a reduced genome responds to foreign DNA but also offer insights into the proper application of these strains. The decreases in growth caused by the cost of carrying foreign DNA were similar for the wild-type and clean-genome strains. A negative correlation between the growth rate and the total amount of exogenous DNA was observed in both strains, but a better theoretical fit with a higher statistical significance was found for the strain with the clean genome. Compared to the wild-type strain, the clean-genome strain exhibited a reduced carrying capacity for exogenous DNA, which was largely attributed to its ability to restrict the replication of foreign DNA. A tendency to allocate energy and resources toward gene expression, but not DNA replication, was observed in the strain with the clean genome. The possession of a clean genome constrained the plasmid copy number to a wild-type-equivalent load. The results indicate that the wild-type strain possesses a greater tolerance for foreign DNA, as in endosymbiosis, and that the use of strains with clean genomes will be favorable in the applications that require precise control and theoretical prediction.
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影响因子：
11
作者：
Haft, Rembrandt J. F.;Mittler, John E.;Traxler, Beth
通讯作者：
Traxler, Beth
影响因子：
2.1
作者：
Harrison，E.;Koufopanou，V.;Maclean，R. C.
通讯作者：
Maclean，R. C.
影响因子：
4.1
作者：
Lee, Changsoo;Kim, Jaai;Hwang, Seokhwan
通讯作者：
Hwang, Seokhwan
影响因子：
3.8
作者：
LEE, SB;SERESSIOTIS, A;BAILEY, JE
通讯作者：
BAILEY, JE
影响因子：
6.4
作者：
Lee JH;Sung BH;Kim MS;Blattner FR;Yoon BH;Kim JH;Kim SC
通讯作者：
Kim SC