Analysis of Rumen Methanogen Diversity in Water Buffaloes (Bubalus bubalis) Under Three Different Diets
Analysis of Rumen Methanogen Diversity in Water Buffaloes (Bubalus bubalis) Under Three Different Diets
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DOI：
10.1007/s00248-012-0007-0
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发表时间：
2012-07-01
影响因子：
3.6
通讯作者：
Wright, Andre-Denis G.
中科院分区：
文献类型：
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作者：
Franzolin, Raul;St-Pierre, Benoit;Wright, Andre-Denis G.
布法罗（Bubalus bubalis）是许多国家生产奶和肉的重要牲畜品种。我们调查了不同的饮食：玉米青贮饲料，放牧牧场，甘蔗在巴西保持的地中海水牛瘤胃产甲烷菌的多样性。从三个产甲烷菌16S rRNA基因克隆文库中分离出总共467个克隆，每个克隆代表不同的饲料类型。467个克隆被分配到19个物种水平的操作分类单位（OTU）。四个OTU在所有三个文库中均有代表，八个OTU是文库特异性的，六个OTU仅在玉米青贮饲料和牧草放牧文库中发现，一个OTU仅在牧草放牧和甘蔗文库之间共享。我们发现，Methanobrevibium相关序列是最丰富的水牛采样我们的分析，在以前报道的研究表明，Methanomicrobium移动样产甲烷菌是最丰富的产甲烷菌在水牛的Murrah和Surti品种在印度采样。考虑到水牛的全球分布和可能提供的各种饮食，我们的研究结果与其他研究组的研究相结合，支持对这种牲畜物种的微生物组进行更大范围的分析，将为地理位置，品种和饮食在确定瘤胃微生物的种群结构方面的贡献提供很大的见解。
The water buffalo (Bubalus bubalis) is a prominent livestock species for the production of milk and meat in many countries. We investigated the diversity of rumen methanogens in Mediterranean water buffaloes maintained in Brazil under different diets: corn silage, grazing pasture, or sugar cane. A total of 467 clones were isolated from three methanogen 16S rRNA gene clone libraries that each represented a distinct feed type. The 467 clones were assigned to 19 species-level operational taxonomic units (OTUs). Four OTUs were represented in all three libraries, eight OTUs were library-specific, six OTUs were found in only the corn silage and pasture grazing libraries, and one OTU was shared only between pasture grazing and sugar cane libraries. We found that Methanobrevibacter-related sequences were the most abundant in the water buffaloes sampled for our analysis, in contrast to previously reported studies showing that Methanomicrobium mobile-like methanogens were the most abundant methanogens in water buffaloes of Murrah and Surti breeds sampled in India. Considering the worldwide distribution of water buffaloes and the likely wide variety of diets provided, our results combined with studies from other groups support that larger scope analyses of microbiomes for this livestock species would provide great insight into the contribution of geographical location, breed, and diet in determining the population structure of rumen microorganisms.