Cryptosporidium ryanae n. sp (Apicomplexa: Cryptosporidiidae) in cattle (Bos taurus)
Cryptosporidium ryanae n. sp (Apicomplexa: Cryptosporidiidae) in cattle (Bos taurus)
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DOI：
10.1016/j.vetpar.2008.05.024
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发表时间：
2008-10-01
影响因子：
2.6
通讯作者：
Trout, James M.
中科院分区：
文献类型：
--
作者：
Fayer, Ronald;Santin, Monica;Trout, James M.
本文报道了从牛体内分离的隐孢子虫一新种--Ryanae隐孢子虫。C. ryanae，先前被鉴定为鹿样隐孢子虫基因型并记录在GenBank中（AY 587166、EU 203216、DQ 182597、AY 741309和DQ 871345），与微小隐孢子虫和牛隐孢子虫相似，但较小。据报道，这种基因型在全世界的牛中普遍存在。本研究从小牛获得的卵囊是哺乳动物中报道的最小的隐孢子虫卵囊，测量为2.94-4.41 μ m，x 2.94-3.68 μ m（平均值= 3.16 μ m x 3.73 μ m），长/宽形状指数为1.18（n = 40）。2头未感染隐孢子虫的犊牛饲喂C ryanae卵囊后，其开放前期为11 d，开放期为15-17 d。卵囊对BALB/c小鼠和羔羊无感染性。纯化PCR扩增的SSU-rDNA、HSP-70和actin基因片段，并对PCR产物进行测序。对三个非连锁位点的多位点分析表明，新物种与所有其他物种不同，也表明缺乏重组，为物种地位提供了进一步的证据。根据形态学、分子生物学等方面的资料，这种仅在普通牛中发现的地理分布广泛的寄生虫被认为是一个新种，并命名为C。瑞安。由爱思唯尔公司出版
A new species, Cryptosporidium ryanae, is described from cattle. Oocysts of C. ryanae, previously identified as the Cryptosporidium deer-like genotype and recorded as such in GenBank (AY587166, EU203216, DQ182597, AY741309, and DQ871345), are similar to those of Cryptosporidium parvum and Cryptosporidium bovis but smaller. This genotype has been reported to be prevalent in cattle worldwide. Oocysts obtained from a calf for the present study are the smallest Cryptosporidium oocysts reported in mammals, measuring 2.94-4.41 mu m, x 2.94-3.68 mu m (mean = 3.16 mu m x 3.73 mu m) with a length/width shape index of 1.18 (n = 40). The pre-patent period for two Cryptosporidium-naive calves fed C ryanae oocysts was 11 days and the patent period was 15-17 days. Oocysts were not infectious for BALB/c mice or lambs. Fragments of the SSU-rDNA, HSP-70, and actin genes amplified by PCR were purified and PCR products were sequenced. Multi-locus analysis of the three unlinked loci demonstrated the new species to be distinct from all other species and also demonstrated a lack of recombination, providing further evidence of species status. Based on morphological, molecular and biological data, this geographically widespread parasite found only in Bos taurus calves is recognized as a new species and is named C. ryanae. Published by Elsevier B.V.