Increased AURKA promotes cell proliferation and predicts poor prognosis in bladder cancer
Increased AURKA promotes cell proliferation and predicts poor prognosis in bladder cancer
复制标题
AURKA 增加可促进细胞增殖并预测膀胱癌的不良预后
DOI：
10.1186/s12918-018-0634-2
复制
发表时间：
2018-12-14
影响因子：
--
通讯作者：
Deng, Youping
中科院分区：
文献类型：
--
作者：
Guo, Mengjie;Lu, Sicheng;Deng, Youping
膀胱癌（Bladder cancer，BC）是膀胱及上尿路最常见的恶性肿瘤，临床治疗受到限制。AURKA（极光激酶A）已被确定为癌基因在癌症的发展，但是，其潜在的作用和潜在的机制在BC的进展仍然unknow.ResultsIn这项研究中，我们评估极光激酶A（AURKA）的表达在患者样本进行基因表达谱，并发现AURKA的表达水平显着高于BC组织比正常组织。BC中AURKA增加与分期和分级密切相关。此外，AURKA升高的BC患者的总体生存率较差。体外实验采用基因过表达和基因沉默的方法，全面验证了AURKA在促进BC细胞增殖中的关键作用。此外，我们证明，AURKA抑制剂MLN8237逮捕BC细胞的生长和诱导apoptosis.ConclusionsThese findings implement AURKA作为一个有效的生物标志物BC检测和预后，以及治疗靶点。
BackgroundBladder cancer (BC) is the most common cancer of the urinary bladder and upper tract, in which the clinical management is limited. AURKA (aurora kinase A) has been identified as an oncogene in cancer development; however, its potential role and underlying mechanisms in the progression of BC remain unknown.ResultsIn this study, we evaluated Aurora kinase A (AURKA) expression in patient samples by performing gene expression profiling, and found that AURKA expression levels were significantly higher in BC tissues than in normal tissues. Increased AURKA in BC was strongly associated with stage and grade. Moreover, BC patients with elevated AURKA achieved poor overall survival rates. The experiments in vitro comprehensively validated the critical role of AURKA in promoting BC cell proliferation using the methods of gene overexpression and gene silencing. Furthermore, we proved that AURKA inhibitor MLN8237 arrested BC cell growth and induced apoptosis.ConclusionsThese findings implicate AURKA acting as an effective biomarker for BC detection and prognosis, as well as therapeutic target.