Seventeen complementation groups of mutations decreasing meiotic recombination in Schizosaccharomyces pombe.
Seventeen complementation groups of mutations decreasing meiotic recombination in Schizosaccharomyces pombe.
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发表时间：
1992-02
期刊：
影响因子：
3.3
通讯作者：
L. DeVeaux;Nicole A. Hoagland;Gerald R. Smith
中科院分区：
文献类型：
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作者：
L. DeVeaux;Nicole A. Hoagland;Gerald R. Smith
我们分析了43个减少裂糖菌减数分裂基因内重组的隐性突变。这些突变是通过筛选ade6位点的染色体重组减少质粒分离出来的。其中16个突变定义了10个新的互补基团，使鉴定的参与减数分裂重组的基因数量达到17个。根据涉及ade6-M26重组热点的杂交中重组缺陷的严重程度，这些突变被分为三个离散的类别。I类突变导致在ade6位点m26刺激的基因内重组至少减少1000倍。II类突变使m26刺激的重组减少了大约100倍。III类突变导致m26刺激或非热点重组减少3-10倍。我们获得了I类和II类突变的多个等位基因，这表明我们可能接近这类突变的饱和。作为定位的第一步，我们使用有丝分裂分离将14个rec基因分配到染色体上。6个rec基因的突变也导致了ura4位点基因内重组的减少；其中5个突变还减少了pro2和arg3基因之间的基因间重组。这些结果表明，这些多重rec基因产物是pombe基因组中高水平减数分裂重组所必需的。
We have analyzed 43 recessive mutations reducing meiotic intragenic recombination in Schizosaccharomyces pombe. These mutations were isolated by a screen for reduced plasmid-by-chromosome recombination at the ade6 locus. Sixteen of the mutations define 10 new complementation groups, bringing to 17 the number of genes identified to be involved in meiotic recombination. The mutations were grouped into three discrete classes depending on the severity of the recombination deficiency in crosses involving the ade6-M26 recombination hotspot. Class I mutations caused at least a 1000-fold reduction in M26-stimulated intragenic recombination at the ade6 locus. Class II mutations reduced M26-stimulated recombination approximately 100-fold. Class III mutations caused a 3-10-fold reduction in either M26-stimulated or non-hotspot recombination. We obtained multiple alleles of class I and class II mutations, suggesting that we may be nearing saturation for mutations of this type. As a first step toward mapping, we used mitotic segregation to assign fourteen of the rec genes to chromosomes. Mutations in the six rec genes tested also caused a decrease in intragenic recombination at the ura4 locus; five of these mutations also reduced intergenic recombination between the pro2 and arg3 genes. These results indicate that these multiple rec gene products are required for high level meiotic recombination throughout the S. pombe genome.