Wool/Acrylic Blended Fabrics as Next-Generation Photodynamic Antimicrobial Materials
Wool/Acrylic Blended Fabrics as Next-Generation Photodynamic Antimicrobial Materials
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羊毛/腈纶混纺面料作为下一代光动力抗菌材料
DOI：
10.1021/acsami.9b09625
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发表时间：
2019-08-21
影响因子：
9.5
通讯作者：
Wang, Qingqing
中科院分区：
文献类型：
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作者：
Chen, Wangbingfei;Chen, Jiang;Wang, Qingqing
采用自消毒材料来减少医院和相关医疗机构中的感染传播受到可扩展的、具有成本效益的和有效的抗菌纺织品的阻碍。在这里，我们研究了是否光动力材料，包括光敏剂嵌入羊毛/丙烯酸类共混物能够介导的光动力灭活革兰氏阳性和革兰氏阴性细菌。构建了羊毛/丙烯酸（W/A）共混织物的小型库，其中羊毛纤维嵌入有作为光敏剂的孟加拉玫瑰红（RB），并且丙烯酸纤维用传统的阳离子黄色X-8 GL染料染色，从而能够实现比用单一光敏剂可实现的更宽的调色板。通过物理（SEM、DSC、TGA、拉伸强度）、光谱（荧光）、比色（K/S和CIELab值）和色牢度（耐摩擦、洗涤）研究来表征所得光动力材料，并且它们对模型底物碘化钾的光氧化证明了这些材料产生杀微生物活性氧物质（即，单线态氧）。我们的最佳结果产生了革兰氏阳性S.金黄色葡萄球菌（99.98%）和B.枯草杆菌（99.993%）在用可见光（60分钟; 65 +/-5 mW/cm（2）; λ>= 420 nm）照射时以类似于4个对数单位增加，尽管观察到对革兰氏阴性铜绿假单胞菌和大肠杆菌更适度的活性。大肠杆菌（1-2 log单位病原体减少）。虽然通过顺序或同时染色步骤生产的双重染色材料没有统计学显著差异，但注意到高负载量的阳离子黄X-8 GL染料确实抑制了RB光敏剂的抗微生物活性，双重染色材料能够介导对S.金黄色葡萄球菌在1% o.w.f X-8 GL负载下。这些研究结果表明，双染色材料的抗微生物光动力失活是独立的染色过程本身，但表现出的局限性加载的传统染料方面的光敏剂的活性。总之，结果表明，光敏剂嵌入混纺织物通过一步染色工艺生产的可行性，作为一种低成本和可扩展的方法，用于创建有效的自消毒纺织品，用于预防感染，其包括第二种传统染料的颜色变化将进一步有利于它们的采用从商业角度来看。
The adoption of self-sterilizing materials to reduce infection transmission in hospitals and related healthcare facilities has been hampered by the availability of scalable, cost-effective, and potent antimicrobial textiles. Here, we investigated whether photodynamic materials comprising photosensitizer-embedded wool/acrylic blends were able to mediate the photodynamic inactivation of Gram-positive and Gram-negative bacteria. A small library of wool/acrylic (W/A) blended fabrics was constructed wherein the wool fibers were embedded with rose Bengal (RB) as a photosensitizer and the acrylic fibers were dyed with a traditional cationic yellow X-8GL dye, thereby enabling a broader color palette than was achievable with a single photosensitizer. The resultant photodynamic materials were characterized by physical (SEM, DSC, TGA, tensile strength), spectroscopic (fluorescence), colorimetric (K/S and CIELab values), and color fastness (against rubbing, washing) studies, and their photooxidation of the model substrate potassium iodide demonstrated the ability of these materials to generate microbicidal reactive oxygen species (i.e., singlet oxygen) upon illumination. Our best results yielded the photodynamic inactivation of Gram-positive S. aureus (99.98%) and B. subtilis (99.993%) by similar to 4 log units upon illumination with visible light (60 min; 65 +/- 5 mW/cm(2); lambda >= 420 nm), although more modest activity was observed against Gram-negative P. aeruginosa and E. coli (1-2 log units pathogen reduction). While there were no statistically significant differences for dual-dyed materials that were produced through either sequential or simultaneous dyeing steps, it was noted that high loadings of the cationic yellow X-8GL dye did inhibit the antimicrobial activity of the RB photosensitizer, with the dual-dyed materials able to mediate a 2.9 log unit reduction against S. aureus at a 1% o.w.f X-8GL loading. These findings indicate that the antimicrobial photodynamic inactivation of dual-dyed materials is independent of the dyeing process itself, yet exhibits limitations on the loading of the traditional dye with regards to the activity of the photosensitizer. Taken together, the results suggest the feasibility of photosensitizer-embedded blended fabrics produced through a one-step dyeing process as a low-cost and scalable method for creating effective self-disinfecting textiles for infection prevention, and whose inclusion of a second traditional dye for color variation will further benefit their adoption from a commercial standpoint.