Synergistic effect of RhBMP-2 and bFGF on ectopic osteogenesis in mice
Synergistic effect of RhBMP-2 and bFGF on ectopic osteogenesis in mice
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DOI：
10.1016/s1995-7645(14)60187-5
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发表时间：
2015-01-01
影响因子：
3.1
通讯作者：
Yin, Zhong-Da
中科院分区：
文献类型：
--
作者：
Ma, Shu-Yuan;Feng, Zhi-Qiang;Yin, Zhong-Da
目的：探讨重组人骨形态发生蛋白2（rhBMP-2）与碱性成纤维细胞生长因子（bFGF）联合应用的协同效应及机制。方法：将24只KM雄性小鼠随机分为6组，每组4只，即A组（对照组）、B组（仅用胶原蛋白处理）、C组（用2 ng bFGF+胶原蛋白处理）、D组（用4 μg rhBMP-2+胶原蛋白处理）、E组（用4 μg rhBMP-2+2 ng bFGF+胶原蛋白处理）和F组（用4 μg rhBMP-2+胶原蛋白处理）。 rhBMP-2+4ng bFGF+胶原植入小鼠后腿肌间隔，对照组则分离小鼠肌间隔，不进行一般观察、钙含量检测、显微CT、三维重建扫描、骨密度（BMD）、骨体积分数（BVF）和小梁厚度（Tb.Th）的测量。结果：D、E、F组小鼠均出现异位成骨，Micro-CT及三维重建显示异位成骨外表面有连续的新生骨物质，骨形成中心未见明显的骨物质充填。 Tb.Th 在 D 组和 E 组或 F 组之间具有统计学显着性（P
Objective: To investigate the synergistic effect and mechanism of the combined application of recombinant human bone morphogenetic protein-2 (rhBMP-2) and basic fibroblast growth factor (bFGF). Methods: 24 KM male mice were randomly divided into 6 groups with 4 mice in each group, namely, Group A (control group), Group B (only treated with collagen), Group C (treated with 2 ng bFGF+collagen), Group D (treated with 4 mu g rhBMP-2+collagen), Group E (treated with 4 mu g rhBMP-2+2 ng bFGF+collagen) and Group F (treated with 4 mu g rhBMP-2+4 ng bFGF+collagen. The composites were implanted into the intermuscular septum of hind legs mice; whereas in control group, intermuscular septum of mice was separated and no implantation was performed. General observation, detection of concentration of calcium content, micro computed tomography (Micro-CT), three-dimensional reconstruction scan, measurement of bone mineral density (BMD), bone volume fraction (BVF) and trabecular thickness (Tb.Th), as well as histological observation with HE staining and ALP and CD34 immumohistochemical staining were performed. Results: Ectopie osteogenesis was found in Groups D, E and F mice. The difference in concentration of calcium contents was statistically significant between Groups D and E (P0.05). Micro-CT and three dimensional reconstruction revealed continuous newborn bone substance in external surface of ectopic bone formation, and the center of bone formation did not show obvious substantial filling by bone substance. The differences in BMD, BVF and Tb.Th were statistically significant between Groups D and E or F (P