Decolorization of azo dyes with immobilized Pseudomonas luteola
Decolorization of azo dyes with immobilized Pseudomonas luteola
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DOI：
10.1016/s0032-9592(00)00274-0
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发表时间：
2001-03-01
影响因子：
4.4
通讯作者：
Chen, SY
中科院分区：
文献类型：
--
作者：
Chang, JS;Chou, C;Chen, SY
利用固定化能酶促还原偶氮基团的藤黄假单胞菌作为偶氮染料废水脱色的生物催化剂。通过在天然和合成聚合物基质中包埋来固定藤黄假单胞菌的细胞。操作条件的影响（例如，温度、pH、染料浓度等）对微生物脱色的影响。重复批式脱色实验评价了固定化生物催化剂的可重复使用性。与悬浮细胞相比，固定化细胞对搅拌速率（溶解氧水平）和pH值不太敏感，而温度对悬浮细胞和固定化细胞的影响相似。用米氏动力学描述了脱色率与染料浓度的表观相关性。经过四次反复实验。游离细胞的脱色率下降了近45%，而CA-、CGN-和PAA-固定化细胞分别保留了其原始活性的75%、85%和80%以上。CGN和PAA固定化细胞在4个循环中达到50%转化率所需的时间（t（1/2））几乎保持相同，表明这些固定化细胞的稳定脱色效率。(C)2001爱思唯尔科技有限公司版权所有。
Immobilized Pseudomonas luteola able to reduce azo groups enzymically were used as a biocatalyst for the decolorization of wastewater containing azo dyes. Cells of P. luteola were immobilized by entrapment in natural and synthetic polymeric matrices. The effects of operational conditions (e.g., temperature, pH, dye concentration, etc.) on microbial decolorization were investigated. The reusability of the immobilized biocatalyst was evaluated with repeated-batch decolorization experiments. Immobilized cells were less sensitive to agitation rates (dissolved oxygen levels) and pH as compared with suspended cells, while the effects of temperature were similar for both suspended and immobilized cells. Michaelis-Menten kinetics was used to describe the apparent correlation between the decolorization rate and the dye concentration. After four repeated experiments. the decolorization rate of the free cells decreased by nearly 45%, while CA-, CGN-, and PAA-immobilized cells retained over 75, 85, and 80% of their original activity, respectively. The time required for 50%, conversion (t(1/2)) remained nearly the same for CGN- and PAA-immobilized cells during four cycles, indicating the stable decolorization efficiency of these immobilized cells. (C) 2001 Elsevier Science Ltd. All rights reserved.