Estimation of angiogenesis with anti-CD105 immunostaining in the process of colorectal cancer development
Estimation of angiogenesis with anti-CD105 immunostaining in the process of colorectal cancer development
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DOI：
10.1067/msy.2002.119361
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发表时间：
2002-01-01
期刊：
影响因子：
3.8
通讯作者：
Abe, T
中科院分区：
文献类型：
--
作者：
Akagi, K;Ikeda, Y;Abe, T
背景泛内皮标记物（如CD 34、CD 31和因子VIII）的表达并不总是在血管生成血管中观察到，并且这些标记物对于测量血管生成是无用的。相反，CD 105优先在血管生成血管中表达，因此可能对测量血管生成有价值。我们假设通过CD 105进行微血管定量可能有助于测量结直肠腺瘤-癌序列中的血管生成。应用单克隆抗体CD 34和CD 105对54例结直肠腺瘤和20例结直肠癌进行免疫组化染色，并在200倍放大下计数微血管密度（MVD）。CD 34阳性的微血管在腺瘤中几乎均匀分布。与此相反，CD 105阳性的微血管优先观察到在腺瘤的表面区域。在癌中，CD 34仅染色一部分CD 105阳性的血管。CD 34在结直肠腺瘤-癌序列中的MVD无显著性差异。与此相反，从低度异型增生到高度异型增生（P <0.0001）和从高度异型增生到癌（P <0.05），CD 105的MVD增加有统计学意义。因此，用CD 105评估结直肠癌发展过程中的新血管形成可能是预测结直肠癌发展风险的有价值的标志物。
Background. The expression of panendothelial markers (eg, CD34, CD31, and factor VIII) is not always Observed in angiogenic vessels, and such markers are not useful for measuring angiogenesis. In contrast, CD105 is preferentially expressed in angiogenic vessels and thus may be, valuable for measuring, angiogenesis. We hypothesized that microvessel quantification by means of CD105 might be useful for measuring angiogenesis in the colorectal adenoma-carcinoma sequence.Methods. We immunohistochemically investigated 54 cases of colorectal adenoma and 20 cases of carcinomas using monoclonal antibodies CD34 and CD105, and microvessel density (MVD) was counted at x200 magnification.Results. Microvessels positive for CD34 were distributed almost uniformly in adenomas. In contrast, microvessels positive for CD105 were preferentially observed in the surface area of adenomas. In carcinomas, CD34 stained only a proportion of blood vessels that were positive for CD105. No significant difference of MVD for CD34 was observed in the colorectal adenoma-carcinoma sequence. In contrast, an increment of MVD for CD105 from low-grade to high-grade dysplasia (P < .0001) and that from high-grade dysplasia to carcinomas (P < .05) was statistically significant.Conclusions. Assessing neovascularization with CD105 in the process of colorectal cancer development may thus be a valuable marker for predicting the risk of colorectal cancer development.