Mesoporous silica nanoparticles-assisted ruthenium(II) complexes for live cell staining
Mesoporous silica nanoparticles-assisted ruthenium(II) complexes for live cell staining
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用于活细胞染色的介孔二氧化硅纳米粒子辅助钌(II)络合物
DOI：
10.1007/s11426-016-0409-4
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发表时间：
2017
影响因子：
9.6
通讯作者：
Sun Shiguo
中科院分区：
文献类型：
--
作者：
Wen Jia;Yan Hui;Xia Pengyi;Xu Yongqian;Li Hongjuan;Sun Shiguo
钌配合物可以通过静电和嵌入作用与DNA结合并产生强发光，已成为DNA染色的理想候选物。但有些化合物如Ru（phen）_3Cl_2和Ru（phen）_2（dppz）Cl_2很难穿过活细胞的细胞膜，限制了它们与活细胞中DNA的进一步相互作用。为了解决这个问题，一个潜在的方法是找到一个合适的载体装载和交付这些钌配合物到活细胞。介孔二氧化硅纳米粒子（MSNs）具有无毒和良好的生物相容性，是很好的候选者。更重要的是，带正电荷的钌配合物可以通过静电引力负载在带负电荷的MSNs上，形成MSNs-Ru杂交物，体外实验表明MSNs对Ru配合物与DNA的相互作用没有任何副作用。此外，发现MSNs-Ru杂交物可以进入活的人类宫颈癌细胞HeLa并对DNA进行染色，而对照实验中相应的钌配合物单独几乎无法穿过细胞膜，这表明MSNs可以用作高效的钌配合物递送纳米材料用于活细胞染色。
Ruthenium complexes which can bind to DNA via electrostatic and intercalation interactions producing strong luminescence have become ideal candidates for DNA staining. However, some of them such as Ru(phen)3Cl2and Ru(phen)2(dppz)Cl2could hardly cross the cellular membrane of live cells which limited their further interaction with DNA in live cells. To solve this problem, a potential approach is to find a proper vehicle for loading and delivery of these ruthenium complexes into live cells. Mesoporous silica nanoparticles (MSNs) with non-toxicity and good biocompatibility can be good candidates. More importantly, ruthenium complexes with positively charge could be loaded on negatively charged MSNs via electrostatic attractions to form MSNs-Ru hybrid.In vitrotest demonstrated that MSNs had no side effects on the interactions between Ru complexes and DNA. Furthermore, it is found that the MSNs-Ru hybrid can enter into living human cervical cancer cells HeLa and stain the DNA while the corresponding ruthenium complexes alone could hardly cross the cellular membrane in the control experiment, demonstrating MSNs can be employed to be an efficient ruthenium complexes delivery nanomaterial for live cell staining.